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首页> 美国卫生研究院文献>Oncotarget >Common housekeeping proteins are upregulated in colorectal adenocarcinoma and hepatocellular carcinoma making the total protein a better housekeeper
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Common housekeeping proteins are upregulated in colorectal adenocarcinoma and hepatocellular carcinoma making the total protein a better housekeeper

机译：普通管家蛋白在大肠腺癌和肝细胞癌中上调使总蛋白成为更好的管家

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摘要

Housekeeping proteins are essential endogenous controls for normalization as they are expected to be stably expressed. However, the stability of the expression level of housekeeping proteins needs to be assessed considering various experimental conditions. Our study evaluated the degree of variability of 7 commonly used housekeeping proteins with regard to their potential utility as normalizers in 56 pairs of matched colorectal adenocarcinoma (CRC) tissue samples and 6 pairs of hepatocellular carcinoma (HCC) tissue samples using multiple reaction monitoring (MRM) and Western blot analyses. A comprehensive experimental design and strict statistical analysis revealed that the expression levels of these 7 housekeeping proteins were not as stable as expected and they all exhibited upregulations to varying degrees in both the CRC and the HCC tissue samples. Consequently, we verified that using the amount of total protein instead of that of an individual protein can serve as a preferable control for studies of protein expression that require normalization.
机译：管家蛋白是正常化必不可少的内源性对照，因为它们有望稳定表达。但是，需要考虑各种实验条件来评估管家蛋白表达水平的稳定性。我们的研究使用多反应监测（MRM）评估了56种配对大肠腺癌（CRC）组织样本和6对肝细胞癌（HCC）组织样本中7种常用管家蛋白作为标准化剂的潜在效用的可变性程度）和Western印迹分析。全面的实验设计和严格的统计分析表明，这7种管家蛋白的表达水平不如预期的稳定，并且它们在CRC和HCC组织样品中均表现出不同程度的上调。因此，我们验证了使用总蛋白量而不是单个蛋白量可以作为需要标准化的蛋白表达研究的优选对照。

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