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首页> 美国卫生研究院文献>The Onderstepoort Journal of Veterinary Research >Virulence gene profiles of avian pathogenic Escherichia coli isolated from chickens with colibacillosis in Bulawayo Zimbabwe
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Virulence gene profiles of avian pathogenic Escherichia coli isolated from chickens with colibacillosis in Bulawayo Zimbabwe

机译：津巴布韦布拉瓦约从患有大肠杆菌病的鸡中分离到的禽致病性大肠杆菌的毒力基因谱

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摘要

Colibacillosis, a disease caused by avian pathogenic Escherichia coli (APEC), is one of the main causes of economic losses in the poultry industry worldwide. This study was carried out in order to determine the APEC-associated virulence genes contained by E. coli isolates causing colibacillosis in chickens. A total of 45 E. coli isolates were obtained from the diagnostics and research branch of the Central Veterinary Laboratories, Bulawayo, Zimbabwe. These isolates were obtained from chickens with confirmed cases of colibacillosis after postmortem examination. The presence of the iutA, hlyF, ompT, frz, sitD, fimH, kpsM, sitA, sopB, uvrY, pstB and vat genes were investigated by multiplex polymerase chain reaction (PCR) assay. Of the 45 isolates, 93% were positive for the presence of at least one virulence gene. The three most prevalent virulence genes were iutA (80%), fimH (33.3%) and hlyF (24.4%). The kpsM, pstB and ompT genes had the lowest prevalence, having been detected in only 2.2% of the isolates. All 12 virulence genes studied were detected in the 45 APEC isolates. Virulence gene profiles were constructed for each APEC isolate from the multiplex data. The APEC isolates were profiled as 62.2% fitting profile A, 31.1% profile B and 6.7% profile C. None of the isolates had more than seven virulence genes. Virulence profiles of Zimbabwean APEC isolates are different from those previously reported. Zimbabwean APEC isolates appear to be less pathogenic and may rely on environmental factors and stress in hosts to establish infection.
机译：大肠杆菌病（Colibacillosis）是一种由禽致病性大肠杆菌（APEC）引起的疾病，是全世界家禽业经济损失的主要原因之一。进行这项研究是为了确定引起鸡大肠杆菌病的大肠杆菌分离株所含的APEC相关毒力基因。从津巴布韦布拉瓦约的中央兽医实验室的诊断和研究部门获得了总共45种大肠杆菌分离株。这些分离株是从验尸检查后确诊为大肠杆菌病的鸡中获得的。通过多重聚合酶链反应（PCR）分析了iutA，hlyF，ompT，frz，sitD，fimH，kpsM，sitA，sopB，uvrY，pstB和vat基因的存在。在这45种分离株中，有93％至少有一种毒力基因呈阳性。三个最普遍的毒力基因是iutA（80％），fimH（33.3％）和hlyF（24.4％）。 kpsM， pstB 和 ompT 基因的患病率最低，仅在2.2％的分离物中检测到。在45种APEC分离物中检测到所有12种毒力基因。从多重数据为每个APEC分离物构建毒力基因谱。将APEC分离株的适合度A分为62.2％，B占31.1％，C占6.7％。这些分离株均没有超过7个毒力基因。津巴布韦APEC分离株的毒力谱与以前报道的不同。津巴布韦的APEC分离株似乎病原性较低，可能依赖于环境因素和宿主的压力来建立感染。

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