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Microbial Diversity of Acidic Hot Spring (Kawah Hujan B) in Geothermal Field of Kamojang Area West Java-Indonesia

机译:西爪哇-印度尼西亚卡莫让地区地热场中酸性温泉的微生物多样性

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摘要

Microbial communities in an acidic hot spring, namely Kawah Hujan B, at Kamojang geothermal field, West Java-Indonesia was examined using culture dependent and culture independent strategies. Chemical analysis of the hot spring water showed a characteristic of acidic-sulfate geothermal activity that contained high sulfate concentrations and low pH values (pH 1.8 to 1.9). Microbial community present in the spring was characterized by 16S rRNA gene combined with denaturing gradient gel electrophoresis (DGGE) analysis. The majority of the sequences recovered from culture-independent method were closely related to Crenarchaeota and Proteobacteria phyla. However, detail comparison among the member of Crenarchaeota showing some sequences variation compared to that the published data especially on the hypervariable and variable regions. In addition, the sequences did not belong to certain genus. Meanwhile, the 16S Rdna sequences from culture-dependent samples revealed mostly close to Firmicute and gamma Proteobacteria.
机译:使用依赖于文化和不依赖于文化的策略对位于西爪哇-印度尼西亚的Kamojang地热田的酸性温泉(Kawah Hujan B)中的微生物群落进行了研究。温泉水的化学分析显示出酸性硫酸盐地热活动的特征,该活动包含高硫酸盐浓度和低pH值(pH 1.8至1.9)。通过16S rRNA基因结合变性梯度凝胶电泳(DGGE)分析来表征春季存在的微生物群落。从非培养依赖性方法中回收的大多数序列与Crenarchaeota和Proteobacteria phyla密切相关。然而,Crenarchaeota成员之间的详细比较显示,与已发表的数据相比,尤其是在高变区和可变区上,有些序列变异。另外,这些序列不属于某些属。同时,依赖于培养物的样品的16S Rdna序列显示出大多数接近Firmicute和γ变形杆菌。

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