• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Philosophical Transactions of the Royal Society B: Biological Sciences >Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications
【2h】

Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications

机译：将DNA条形码应用于红色大型藻类：初步评估为将来的应用带来了希望

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Marine macroalgae, especially the Rhodophyta, can be notoriously difficult to identify owing to their relatively simple morphology and anatomy, convergence, rampant phenotypic plasticity, and alternation of heteromorphic generations. It is thus not surprising that algal systematists have come to rely heavily on genetic tools for molecular assisted alpha taxonomy. Unfortunately the number of suitable marker systems in the three available genomes is enormous and, although most workers have settled on one of three or four models, the lack of an accepted standard hinders the comparison of results between laboratories. The advantages of a standard system are obvious for practical purposes of species discovery and identification; as well, compliance with a universal marker, such as cox1 being developed under the label ‘DNA barcode’, would allow algal systematists to benefit from the rapidly emerging technologies. Novel primers were developed for red algae to PCR amplify and sequence the 5′ cox1 ‘barcode’ region and were used to assess three known species-complex questions: (i) Mazzaella species in the Northeast Pacific; (ii) species of the genera Dilsea and Neodilsea in the Northeast Pacific; and (iii) Asteromenia peltata from three oceans. These models were selected because they have all caused confusion with regards to species number, distribution, and identification in the field, and because they have all been studied with molecular tools. In all cases the DNA barcode resolved accurately and unequivocally species identities and, with the enhanced sampling here, turned up a variety of novel observations in need of further taxonomic investigation.
机译：众所周知，海洋大型藻类，尤其是红藻藻类，由于其相对简单的形态和解剖结构，会聚，猖ramp的表型可塑性以及异质世代的交替而难以识别。因此，藻类系统学家已严重依赖于遗传工具进行分子辅助的α分类法也就不足为奇了。不幸的是，三个可用基因组中合适的标记系统数量很多，尽管大多数工作人员都选择了三个或四个模型之一，但缺乏公认的标准阻碍了实验室之间结果的比较。对于物种发现和鉴定的实际目的而言，标准系统的优势显而易见。同样，如果符合通用标记（例如在标签“ DNA条码”下开发的cox1）的兼容，藻类系统学家也可以从迅速兴起的技术中受益。开发了用于红藻的新型引物，用于PCR扩增5'cox1“条形码”区域并对其进行测序，并用于评估三个已知的物种复杂的问题：（i）东北太平洋的马扎氏菌物种； （ii）东北太平洋的迪尔西和新地西属的物种； （iii）来自三大洋的Asteromenia peltata。之所以选择这些模型，是因为它们都在种类，分布和野外识别方面引起了混乱，并且都已经使用分子工具进行了研究。在所有情况下，DNA条形码都能准确，明确地解析物种身份，并且随着此处采样的增加，出现了许多需要进一步分类研究的新颖观察结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号