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首页> 美国卫生研究院文献>Physiology and Molecular Biology of Plants >Biochemical physiological and molecular evaluation of rice cultivars differing in salt tolerance at the seedling stage
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Biochemical physiological and molecular evaluation of rice cultivars differing in salt tolerance at the seedling stage

机译：不同耐盐性水稻品种苗期的生化生理和分子评价

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Changes in the antioxidant enzymes, lipid peroxidation, sodium and potassium, chlorophyll, H2O2 and proline content were monitored in the leaves of 42 rice varieties which were not yet well-documented for the salinity tolerance under different salinity levels. The tolerant varieties (FL478, Hassani, Shahpasand, Gharib and Nemat) showed signs of tolerance (lower Na+/K+ ratio, high proline accumulation, less membrane damage, lower H2O2 production, and higher superoxide dismutase and catalase activity) very well. The positive relationship between the level of salt tolerance and the amount of proline accumulation in the rice varieties support the important role of proline under the salt stress. The varieties were genotyped for 12 microsatellite markers that were closely linked to SalTol QTL. The results of association analysis indicated that RM1287, RM8094, RM3412 and AP3206 markers had the high value of R2 for the regression models of the studied traits. It shows the important role of SalTol in controlling physio-biochemical traits. The results can be used in the future marker assisted selection (MAS) directly, if the results are confirmed. Electronic supplementary materialThe online version of this article (doi:10.1007/s12298-017-0440-0) contains supplementary material, which is available to authorized users.
机译：监测了42个水稻品种叶片中抗氧化酶，脂质过氧化，钠和钾，叶绿素，H2O2和脯氨酸含量的变化，这些文献尚未充分证明不同盐度水平下的耐盐性。耐性品种（FL478，Hassani，Shahpasand，Gharib和Nemat）显示出耐性的迹象（Na + / K + 降低，脯氨酸积累高，膜损伤少，降低H2O2的产生，以及较高的超氧化物歧化酶和过氧化氢酶活性）非常好。水稻品种的耐盐性水平与脯氨酸积累量之间的正相关关系支持了脯氨酸在盐胁迫下的重要作用。对该品种进行了与SalTol QTL密切相关的12个微卫星标记的基因分型。关联分析结果表明，RM1287，RM8094，RM3412和AP3206标记在所研究性状的回归模型中具有较高的R 2 值。它显示了SalTol在控制生理生化特征中的重要作用。如果结果得到确认，则结果可直接用于将来的标记辅助选择（MAS）。电子补充材料本文的在线版本（doi：10.1007 / s12298-017-0440-0）包含补充材料，授权用户可以使用。

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