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首页> 美国卫生研究院文献>Plant Biotechnology >Constitutive activation of plant-specific RAB5 GTPase confers increased resistance against adapted powdery mildew fungus
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Constitutive activation of plant-specific RAB5 GTPase confers increased resistance against adapted powdery mildew fungus

机译：植物特异性RAB5 GTPase的组成性激活赋予增强的抗白粉病真菌抗性

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The obligate biotrophic fungal pathogens that cause powdery mildew disease establish infection in living host cells by modifying host cellular functions, including membrane trafficking. Previously, we reported that two Arabidopsis thaliana RAB5 GTPases, plant-specific ARA6/RABF1 and canonical ARA7/RABF2b, accumulate at the extrahaustorial membrane (EHM), which surrounds the specialized infection hypha called the haustorium. In this study, we examined the role of ARA6 and ARA7, which regulate distinctive endosomal trafficking pathways, in plant–powdery mildew fungus interactions. Although ARA6- and ARA7-related mutants did not exhibit altered susceptibility to the A. thaliana–adapted powdery mildew fungus Golovinomyces orontii, overexpression of constitutively active ARA6, but not constitutively active ARA7, repressed proliferation of G. orontii. The repression of fungal proliferation was associated with accelerated formation of the callosic encasement around the haustorium. Furthermore, microscopic observation revealed an accumulation of the constitutively active form of ARA6, but not active ARA7, at the EHM. These results indicate that plant-specific ARA6 has a specific role in plant–powdery mildew fungus interaction, and manipulation of ARA6 activity could be a novel tool to overcome this plant disease.
机译：引起白粉病的专性生物营养真菌病原体通过修饰宿主细胞功能（包括膜运输）在活宿主细胞中建立感染。以前，我们报道了两个拟南芥RAB5 GTPases，植物特有的ARA6 / RABF1和典范ARA7 / RABF2b，聚集在围绕着专门的感染菌丝被称为haustorium的体外膜（EHM）上。在这项研究中，我们检查了ARA6和ARA7在调节植物-粉状霉菌相互作用中的作用，它们调节独特的内体运输途径。尽管与ARA6-和ARA7相关的突变体对拟南芥白粉病真菌Golovinomyces orontii的敏感性没有改变，但组成型有活性的ARA6的过表达而不是组成型有活性的ARA7抑制了拟南芥的增殖。真菌增殖的抑制与在化脓室周围愈伤组织包裹物的加速形成有关。此外，显微镜观察显示在EHM处有ARA6组成性活性形式的积累，但没有ARA7活性。这些结果表明，植物特有的ARA6在植物-粉状霉菌的相互作用中具有特定的作用，并且操纵ARA6活性可能是克服这种植物病害的一种新颖工具。

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