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首页> 美国卫生研究院文献>The Plant Cell >A novel extensin gene encoding a hydroxyproline-rich glycoprotein requires sucrose for its wound-inducible expression in transgenic plants.
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A novel extensin gene encoding a hydroxyproline-rich glycoprotein requires sucrose for its wound-inducible expression in transgenic plants.

机译：编码富含羟脯氨酸的糖蛋白的新型延伸蛋白基因需要蔗糖才能在转基因植物中诱导伤口表达。

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摘要

A novel hydroxyproline-rich glycoprotein (SbHRGP3) that consists of two different domains is encoded by an extensin gene from soybean. The first domain (domain 1) located at the N terminus is composed of 11 repeats of Ser-Pro4-Lys-His-Ser-Pro4-Tyr3-His, whereas the second domain (domain 2) at the C terminus contains five repeats of Ser-Pro4-Val-Tyr-Lys-Tyr-Lys-Ser-Pro4-Tyr-Lys-Tyr-Pro-Ser-Pro5-Tyr-Lys-T yr- Pro-Ser-Pro4-Val-Tyr-Lys-Tyr-Lys. These two repeat motifs are organized in an extremely well-ordered pattern in each domain, which suggests that SbHRGP3 belongs to a new group of proteins having the repeat motifs of two distinct groups of dicot extensins. The expression of the SbHRGP3 gene increased with seedling maturation, and its expression was relatively high in the mature regions of the hypocotyl and in the root of soybean seedlings. An SbHRGP3-beta-glucuronidase (SbHRGP3-GUS) chimeric gene was constructed and expressed in transgenic tobacco plants. The expression of the SbHRGP3-GUS gene was not induced by wounding alone in transgenic tobacco plants; sucrose was also required. Expression was specific to phloem tissues and cambium cells of leaves and stems. In transgenic tobacco seedlings, SbHRGP3-GUS gene expression was activated by the maturation of the primary root and then inactivated; however, reactivation was specifically at the epidermis of the zone from which the lateral root was to be initiated. Its reactivation occurred just before the lateral root initiation. These results indicate that the SbHRGP3 gene in different tissues responds to different signals.
机译：由两个不同域组成的新型富含羟基脯氨酸的糖蛋白（SbHRGP3）由大豆的一种extensin基因编码。位于N末端的第一个域（域1）由Ser-Pro4-Lys-His-Ser-Pro4-Tyr3-His的11个重复组成，而位于C末端的第二个域（域2）包含5个重复序列。 Ser-Pro4-Val-Tyr-Lys-Tyr-Lys-Ser-Pro-Pro4-Tyr-Lys-Tyr-Pro-Ser-Pro5-5-Tyr-Lys-Tyr-Pro-Ser-Pro4-Val-Tyr-Lys-Tyr -是的这两个重复基序在每个结构域中以非常有序的模式组织，这表明SbHRGP3属于新的一组蛋白质，该蛋白质具有两个不同的双子叶植物延伸蛋白基团的重复基序。 SbHRGP3基因的表达随幼苗的成熟而增加，在下胚轴的成熟区域和大豆幼苗的根中表达较高。构建了SbHRGP3-β-葡糖醛酸糖苷酶（SbHRGP3-GUS）嵌合基因，并在转基因烟草植物中表达。 SbHRGP3-GUS基因的表达不是在转基因烟草植物中单独受创诱导的。还需要蔗糖。表达对叶和茎的韧皮部组织和形成层细胞具有特异性。在转基因烟草幼苗中，SbHRGP3-GUS基因表达通过初生根成熟而被激活，然后被灭活。然而，重新激活特别是在侧根要从其开始的区域的表皮上进行。它的重新激活发生在侧根开始之前。这些结果表明，不同组织中的SbHRGP3基因对不同信号有反应。

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