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The Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape Formation

机译:拟南芥rol1突变体中的黄酮醇糖基化修饰配置文件导致植物生长和细胞形状形成的变化。

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摘要

Flavonoids are secondary metabolites known to modulate plant growth and development. A primary function of flavonols, a subgroup of flavonoids, is thought to be the modification of auxin fluxes in the plant. Flavonols in the cell are glycosylated, and the repressor of lrx1 (rol1) mutants of Arabidopsis thaliana, affected in rhamnose biosynthesis, have a modified flavonol glycosylation profile. A detailed analysis of the rol1-2 allele revealed hyponastic growth, aberrant pavement cell and stomatal morphology in cotyledons, and defective trichome formation. Blocking flavonoid biosynthesis suppresses the rol1-2 shoot phenotype, suggesting that it is induced by the modified flavonol profile. The hyponastic cotyledons of rol1-2 are likely to be the result of a flavonol-induced increase in auxin concentration. By contrast, the pavement cell, stomata, and trichome formation phenotypes appear not to be induced by the modified auxin distribution. Together, these results suggest that changes in the composition of flavonols can have a tremendous impact on plant development through both auxin-induced and auxin-independent processes.
机译:类黄酮是已知可调节植物生长发育的次生代谢产物。黄酮醇(类黄酮的一个子集)的主要功能被认为是修饰植物中的生长素通量。细胞中的黄酮醇被糖基化,拟南芥lrx1(rol1)突变体在鼠李糖生物合成中的阻遏物具有修饰的黄酮醇糖基化谱。对rol1-2等位基因的详细分析显示,鼻突生长,子叶的异常铺面细胞和气孔形态以及毛状毛形成不良。阻止类黄酮的生物合成抑制rol1-2芽表型,表明它是由修饰的黄酮醇谱诱导的。 rol1-2的鼻下叶子叶可能是黄酮醇诱导的生长素浓度增加的结果。相比之下,路面细胞,气孔和毛状体形成表型似乎不被修饰的生长素分布所诱导。总之,这些结果表明,黄酮醇组成的变化可通过植物生长素诱导的和植物生长素非独立的过程对植物发育产生巨大影响。

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