• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Plant Cell >Jasmonate and ppHsystemin Regulate Key Malonylation Steps in the Biosynthesis of 17-Hydroxygeranyllinalool Diterpene Glycosides an Abundant and Effective Direct Defense against Herbivores in Nicotiana attenuata
【2h】

Jasmonate and ppHsystemin Regulate Key Malonylation Steps in the Biosynthesis of 17-Hydroxygeranyllinalool Diterpene Glycosides an Abundant and Effective Direct Defense against Herbivores in Nicotiana attenuata

机译：茉莉酸和ppHsystemin调节17-羟基香菜兰油基二萜糖苷生物合成中的关键丙二酸化步骤这是一种对烟草中食草动物的丰富而有效的直接防御。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We identified 11 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) that occur in concentrations equivalent to starch (mg/g fresh mass) in aboveground tissues of coyote tobacco (Nicotiana attenuata) and differ in their sugar moieties and malonyl sugar esters (0-2). Concentrations of HGL-DTGs, particularly malonylated compounds, are highest in young and reproductive tissues. Within a tissue, herbivore elicitation changes concentrations and biosynthetic kinetics of individual compounds. Using stably transformed N. attenuata plants silenced in jasmonate production and perception, or production of N. attenuata Hyp-rich glycopeptide systemin precursor by RNA interference, we identified malonylation as the key biosynthetic step regulated by herbivory and jasmonate signaling. We stably silenced N. attenuata geranylgeranyl diphosphate synthase (ggpps) to reduce precursors for the HGL-DTG skeleton, resulting in reduced total HGL-DTGs and greater vulnerability to native herbivores in the field. Larvae of the specialist tobacco hornworm (Manduca sexta) grew up to 10 times as large on ggpps silenced plants, and silenced plants suffered significantly more damage from herbivores in N. attenuata's native habitat than did wild-type plants. We propose that high concentrations of HGL-DTGs effectively defend valuable tissues against herbivores and that malonylation may play an important role in regulating the distribution and storage of HGL-DTGs in plants.
机译：我们鉴定了11种17-羟基香叶香兰素二萜糖苷（HGL-DTGs），它们的浓度相当于土狼烟草（Nicotiana detecta）地上组织中的淀粉（mg / g新鲜质量），并且它们的糖部分和丙二酰糖酯（0- 2）。在年轻和生殖组织中，HGL-DTG的浓度最高，尤其是丙二酰化化合物。在组织内，草食动物的诱变会改变单个化合物的浓度和生物合成动力学。使用在茉莉花生产和感知中沉默的稳定转化的弱毒猪笼草植物，或通过RNA干扰产生弱毒猪笼草富含Hyp的糖肽系统，我们将丙二酰化确定为由草食和茉莉花信号调节的关键生物合成步骤。我们稳定地沉默了减毒猪笼草香叶基Geranylgeranyl二磷酸合酶（ggpps），以减少HGL-DTG骨架的前体，从而导致总HGL-DTG减少，并且更容易受到野外天然草食动物的侵害。在ggpps沉默植物上，专业烟草香虫（Manduca sexta）的幼虫长到原来的10倍，而沉默植物比普通野生植物遭受弱化猪笼草原生生境中食草动物的破坏要大得多。我们建议，高浓度的HGL-DTGs有效地保护有价值的组织免受草食动物的侵害，并且丙二酰化作用可能在调节HGL-DTGs在植物中的分布和储存中起重要作用。

著录项

相似文献

  • 外文文献
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号