• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Plant Cell >A Small-Molecule Screen Identifies l-Kynurenine as a Competitive Inhibitor of TAA1/TAR Activity in Ethylene-Directed Auxin Biosynthesis and Root Growth in Arabidopsis
【2h】

A Small-Molecule Screen Identifies l-Kynurenine as a Competitive Inhibitor of TAA1/TAR Activity in Ethylene-Directed Auxin Biosynthesis and Root Growth in Arabidopsis

机译：一个小分子筛查确定左旋尿嘧啶为拟南芥中乙烯定向生长素生物合成和根系生长中TAA1 / TAR活性的竞争性抑制剂。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The interactions between phytohormones are crucial for plants to adapt to complex environmental changes. One example is the ethylene-regulated local auxin biosynthesis in roots, which partly contributes to ethylene-directed root development and gravitropism. Using a chemical biology approach, we identified a small molecule, l-kynurenine (Kyn), which effectively inhibited ethylene responses in Arabidopsis thaliana root tissues. Kyn application repressed nuclear accumulation of the ETHYLENE INSENSITIVE3 (EIN3) transcription factor. Moreover, Kyn application decreased ethylene-induced auxin biosynthesis in roots, and TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1/TRYPTOPHAN AMINOTRANSFERASE RELATEDs (TAA1/TARs), the key enzymes in the indole-3-pyruvic acid pathway of auxin biosynthesis, were identified as the molecular targets of Kyn. Further biochemical and phenotypic analyses revealed that Kyn, being an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicked the loss of TAA1/TAR functions. Molecular modeling and sequence alignments suggested that Kyn effectively and selectively binds to the substrate pocket of TAA1/TAR proteins but not those of other families of aminotransferases. To elucidate the destabilizing effect of Kyn on EIN3, we further found that auxin enhanced EIN3 nuclear accumulation in an EIN3 BINDING F-BOX PROTEIN1 (EBF1)/EBF2-dependent manner, suggesting the existence of a positive feedback loop between auxin biosynthesis and ethylene signaling. Thus, our study not only reveals a new level of interactions between ethylene and auxin pathways but also offers an efficient method to explore and exploit TAA1/TAR-dependent auxin biosynthesis.
机译：植物激素之间的相互作用对于植物适应复杂的环境变化至关重要。一个例子是根中乙烯调节的局部植物生长素的生物合成，这部分有助于乙烯定向根的发育和向重力性。使用化学生物学方法，我们确定了一种小分子l-犬尿氨酸（Kyn），它可以有效抑制拟南芥根组织中的乙烯反应。 Kyn的应用抑制了乙烯不敏感3（EIN3）转录因子的核积累。此外，Kyn的施用减少了乙烯诱导的根部植物生长素的生物合成，并且植物生长素生物合成的吲哚-3-丙酮酸途径中的关键酶-阿拉伯糖苷1 /酪氨酸氨基转移酶相关的TRYPTOPHAN氨基酸转移酶（TAA1 / TARs）被确定为分子靶标。凯恩进一步的生化和表型分析表明，Kyn是另一种底物，竞争性抑制TAA1 / TAR活性，而Kyn处理模仿了TAA1 / TAR功能的丧失。分子建模和序列比对表明，Kyn有效且选择性地结合TAA1 / TAR蛋白的底物口袋，但不结合其他氨基转移酶家族的底物口袋。为了阐明Kyn对EIN3的破坏作用，我们进一步发现生长素以EIN3结合F-BOX蛋白1（EBF1）/ EBF2依赖的方式增强EIN3核积累，表明生长素生物合成与乙烯信号之间存在正反馈回路。因此，我们的研究不仅揭示了乙烯和植物生长素途径之间相互作用的新水平，而且为探索和利用TAA1 / TAR依赖的植物生长素生物合成提供了一种有效的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号