• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Plant Cell >Identification of LATE BLOOMER2 as a CYCLING DOF FACTOR Homolog Reveals Conserved and Divergent Features of the Flowering Response to Photoperiod in Pea
【2h】

Identification of LATE BLOOMER2 as a CYCLING DOF FACTOR Homolog Reveals Conserved and Divergent Features of the Flowering Response to Photoperiod in Pea

机译：晚期BLOOMER2作为循环自由度因子的鉴定同源物揭示了豌豆开花期对光周期的响应的保守和发散特征。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The molecular pathways responsible for the flowering response to photoperiod have been extensively studied in Arabidopsis thaliana and cereals but remain poorly understood in other major plant groups. Here, we describe a dominant mutant at the LATE BLOOMER2 (LATE2) locus in pea (Pisum sativum) that is late-flowering with a reduced response to photoperiod. LATE2 acts downstream of light signaling and the circadian clock to control expression of the main photoperiod-regulated FT gene, FTb2, implying that it plays a primary role in photoperiod measurement. Mapping identified the CYCLING DOF FACTOR gene CDFc1 as a strong candidate for LATE2, and the late2-1D mutant was found to carry a missense mutation in CDFc1 that impairs its capacity to bind to the blue-light photoreceptor FKF1 in yeast two-hybrid assays and delays flowering in Arabidopsis when overexpressed. Arabidopsis CDF genes are important negative regulators of CONSTANS (CO) transcription, but we found no effect of LATE2 on the transcription of pea CO-LIKE genes, nor on genes in any other families previously implicated in the activation of FT in Arabidopsis. Our results reveal an important component of the pea photoperiod response pathway and support the view that regulation of FTb2 expression by photoperiod occurs via a CO-independent mechanism.
机译：在拟南芥和谷物中已经广泛研究了负责光周期开花反应的分子途径，但在其他主要植物群中仍知之甚少。在这里，我们描述了豌豆（Pisum sativum）的LATE BLOOMER2（LATE2）基因座上的显性突变体，该突变体开花较晚，对光周期的响应降低。 LATE2在光信号和生物钟的下游控制主要受光周期调节的FT基因FTb2的表达，这表明它在光周期测量中起主要作用。映射确定了CYCLING DOF FACTOR基因CDFc1是LATE2的强力候选者，并且在酵母双杂交检测和鉴定中发现late2-1D突变体在CDFc1中带有错义突变，从而削弱了其与蓝光感光体FKF1结合的能力。过表达会延迟拟南芥的开花。拟南芥CDF基因是CONSTANS（CO）转录的重要负调控因子，但我们发现LATE2对豌豆 CO-LIKE 基因的转录没有影响，也没有影响以前与激活有关的任何其他家族的基因拟南芥中的 FT 我们的结果揭示了豌豆光周期反应途径的重要组成部分，并支持这样的观点，即光周期对 FTb2 表达的调节是通过 CO-独立机制发生的。

著录项

相似文献

  • 外文文献
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号