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首页> 美国卫生研究院文献>Plant Physiology >Cytokinins in Vinca rosea L. Crown Gall Tumor Tissue as Influenced by Compounds Containing Reduced Nitrogen
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Cytokinins in Vinca rosea L. Crown Gall Tumor Tissue as Influenced by Compounds Containing Reduced Nitrogen

机译：长春花冠胆肿瘤组织中的细胞分裂素受含氮量降低化合物的影响

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摘要

Several compounds containing reduced nitrogen markedly increased the yields of cell-division compounds extractable from an A6 Vinca rosea L. crown gall tumor tissue. Casein hydrolysate, several amino acids, and ammonium salts were effective. Both trans-zeatin and ribosyl-trans-zeatin were substantially increased in total amount per culture and in concentration. These two compounds have been identified by several criteria including mass spectra. The reduced nitrogen treatments also caused the appearance of a cytokinin not previously detected in this tissue; it has not yet been identified. The tumor tissue rapidly absorbed [8-14C]adenine from a liquid medium. Within 1 hour, the tissue converted some of the adenine to zeatin and ribosylzeatin, and greater degrees of conversion occurred in 2-, 4-, and 8-hour periods. The tissue grown on a medium containing ammonium chloride accumulated considerably greater quantities of the two cytokinins made from the labeled adenine during each incubation period.
机译：几种含氮减少的化合物显着提高了可从A6长春花冠冕冠状肿瘤组织中提取的细胞分裂化合物的产量。酪蛋白水解物，几种氨基酸和铵盐是有效的。反玉米蛋白和核糖基反玉米蛋白的每次培养总量和浓度均显着增加。这两种化合物已通过包括质谱在内的多个标准进行了鉴定。减少的氮处理也导致了这种组织中以前未检测到的细胞分裂素的出现。尚未确定。肿瘤组织从液体介质中迅速吸收[8- 14 C]腺嘌呤。在1小时内，组织将一些腺嘌呤转化为玉米蛋白和核糖基玉米蛋白，并且在2、4和8小时内发生了更大程度的转化。在每个孵育期间，在含有氯化铵的培养基上生长的组织会积累大量的由标记腺嘌呤制成的两种细胞分裂素。

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