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Effects of Inhibitors of Catalase on Photosynthesis and on Catalase Activity in Unwashed Preparations of Intact Chloroplasts

机译:过氧化氢酶抑制剂对未洗涤的完整叶绿体制剂中光合作用和过氧化氢酶活性的影响

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摘要

The catalase activity of unwashed preparations containing intact spinach (Spinacia oleracea L.) chloroplasts is inhibited both by cyanide and by azide at concentrations which also cause inhibition of photosynthetic CO2- dependent O2 evolution.Aminotriazole can also be used to inhibit this contaminant catalase, and in this case inhibition of catalase can be achieved at aminotriazole concentrations which have little effect on the rate of photosynthetic CO2 fixation. Aminotriazole may be used as a specific inhibitor of catalase in order to demonstrate inhibition of photosynthesis by added H2O2.It is therefore concluded that inhibition of photosynthesis by cyanide and azide does not necessarily result from inhibition of catalase in the chloroplast preparation, and that intact chloroplasts do not produce inhibitory concentrations of H2O2 under the best experimental conditions for CO2 fixation.
机译:含有完整菠菜(Spinacia oleracea L.)叶绿体的未洗涤制剂的过氧化氢酶活性受到氰化物和叠氮化物的抑制,其浓度也会导致光合作用CO2依赖性O2的释放受到抑制,氨基三唑也可用于抑制这种污染的过氧化氢酶,以及在这种情况下,可以在氨基三唑浓度下实现对过氧化氢酶的抑制,而该浓度对光合作用的CO2固定速率几乎没有影响。为了证明过氧化氢对光合作用的抑制作用,可以使用氨基三唑作为过氧化氢酶的特异性抑制剂,因此得出结论,氰化物和叠氮化物对光合作用的抑制不一定是叶绿体制剂中对过氧化氢酶的抑制作用以及完整的叶绿体的抑制作用。在固定CO2的最佳实验条件下,不会产生抑制浓度的H2O2。

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