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首页> 美国卫生研究院文献>Plant Physiology >Characteristics of Glutamate Dehydrogenase in Mitochondria Prepared from Corn Shoots
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Characteristics of Glutamate Dehydrogenase in Mitochondria Prepared from Corn Shoots

机译：玉米笋线粒体中谷氨酸脱氢酶的特征

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The amination of α-ketoglutarate (α-KG) by NADH-glutamate dehydrogenase (GDH) obtained from Sephadex G-75 treated crude extracts from shoots of 5-day-old seedlings was stimulated by the addition of Ca2+. The NADH-GDH purified 161-fold with ammonium sulfate, DEAE-Toyopearl, and Sephadex G-200 was also activated by Ca2+ in the presence of 160 micromolar NADH. However, with 10 micromolar NADH, Ca2+ had no effect on the NADH-GDH activity. The deamination reaction (NAD-GDH) was not influenced by the addition of Ca2+.About 25% of the NADH-GDH activity was solubilized from purified mitochondria after a simple osmotic shock treatment, whereas the remaining 75% of the activity was associated with the mitochondrial membrane fraction. When the lysed mitochondria, mitochondrial matrix, or mitochondrial membrane fraction was used as the source of NADH-GDH, Ca2+ had little effect on its activity. The mitochondrial fraction contained about 155 nanomoles Ca per milligram of mitochondrial protein, suggesting that the NADH-GDH in the mitochondria is already in an activated form with regard Ca2+. In a simulated in vitro system using concentrations of 6.4 millimolar NAD, 0.21 millimolar NADH, 5 millimolar α-KG, and 5 millimolar glutamate thought to occur in the mitochondria, together with 1 millimolar Ca2+, 10 and 50 millimolar NH4+, and purified enzyme, the equilibrium of GDH was in the direction of glutamate formation.
机译：通过添加Ca 2+和Ca 2+刺激由Sephadex G-75处理的5天龄幼芽的粗提物中获得的NADH-谷氨酸脱氢酶（GDH）对α-酮戊二酸（α-KG）的胺化。 / sup>。在160微摩尔NADH存在下，Ca 2 + 活化了用硫酸铵，DEAE-Toyopearl和Sephadex G-200纯化161倍的NADH-GDH。然而，使用10微摩尔NADH时，Ca 2 + 对NADH-GDH活性没有影响。脱氨基反应（NAD-GDH）不受添加Ca 2 + 的影响。经过简单的渗透压电击处理后，纯化的线粒体中溶解了约25％的NADH-GDH活性。 75％的活性与线粒体膜部分有关。当溶解的线粒体，线粒体基质或线粒体膜组分用作NADH-GDH的来源时，Ca 2 + 对它的活性影响很小。每毫克线粒体蛋白质的线粒体级分包含约155纳摩尔Ca，这表明线粒体中的NADH-GDH就Ca 2 + 而言已处于活化形式。在模拟的体外系统中，使用浓度为6.4毫摩尔的NAD，0.21毫摩尔的NADH，5毫摩尔的α-KG和5毫摩尔的谷氨酸，以及1毫摩尔的Ca 2 + ，10 50毫摩尔的NH4 + 和纯化的酶，GDH的平衡朝谷氨酸形成的方向。

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