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首页> 美国卫生研究院文献>Plant Physiology >Correct Targeting of the Bean Storage Protein Phaseolin in the Seeds of Transformed Tobacco
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Correct Targeting of the Bean Storage Protein Phaseolin in the Seeds of Transformed Tobacco

机译：转化烟草种子中豆贮藏蛋白菜豆蛋白的正确靶向

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摘要

The storage protein phaseolin accumulates during seed development in protein bodies in cotyledons of the common bean Phaseolus vulgaris. Hall et al. (In L Van Vloten-Doting, TC Hall, eds, Molecular Form and Function of the Plant Genome, 1985 Plenum Press, In press) recently reported the expression of a gene coding for phaseolin and the accumulation of phaseolin protein in developing seeds of tobacco plants regenerated from transformed callus cells. The protein did not accumulate in other organs of the plants. Mature seeds from normal and transformed tobacco plants were obtained and the subcellular distribution of phaseolin in the seeds was examined using both light and electron microscopic immunocytochemical methods. Phaseolin was found in six of seven transformed tobacco embryos examined, but was present in only one endosperm of five. When present, phaseolin was located exclusively in the protein bodies of the embryonic and endospermic cells. Furthermore, phaseolin was restricted solely to the amorphous matrix of the protein bodies and was excluded from the globoid and proteinaceous crystalloid components of these organelles. The subcellular location of phaseolin in seeds from transformed tobacco plants is similar to that seen in mature seeds of the common bean indicating that in the transformed cells the protein is targeted to the right subcellular compartment.
机译：贮藏蛋白菜豆蛋白在种子发育过程中积累在普通菜豆菜豆子叶的蛋白质体中。霍尔等。 （在L Van Vloten-Doting，TC Hall编辑，《植物基因组的分子形式和功能》，1985年，Plenum Press，印刷中）最近报道了编码菜豆蛋白的基因的表达和菜豆蛋白在烟草种子中的积累。从转化的愈伤组织细胞中再生出植物。该蛋白质没有在植物的其他器官中积累。获得了正常和转化烟草植物的成熟种子，并使用光镜和电子显微镜免疫细胞化学方法检查了菜豆蛋白在种子中的亚细胞分布。在检查的七个转化烟草胚中有六个发现了菜豆蛋白，但在五个胚乳中只有一个存在。当存在时，菜豆蛋白仅位于胚胎和胚乳细胞的蛋白质体内。此外，菜豆蛋白仅限于蛋白质体的无定形基质，并从这些细胞器的球状和蛋白质类晶体成分中排除。转化烟草植物种子中菜豆蛋白的亚细胞位置类似于在普通豆成熟种子中看到的位置，这表明在转化细胞中，蛋白质靶向于右亚细胞区室。

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