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首页> 美国卫生研究院文献>Plant Physiology >Isoenzymes of Superoxide Dismutase in Nodules of Phaseolus vulgaris L. Pisum sativum L. and Vigna unguiculata (L.) Walp.
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Isoenzymes of Superoxide Dismutase in Nodules of Phaseolus vulgaris L. Pisum sativum L. and Vigna unguiculata (L.) Walp.

机译：菜豆豌豆和Pi豆结节中的超氧化物歧化酶的同工酶。

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摘要

The activity and isozymic composition of superoxide dismutase (SOD; EC 1.15.1.1) were determined in nodules of Phaseolus vulgaris L., Pisum sativum L., and Vigna unguiculata (L.) Walp. formed by Rhizobium phaseoll 3622, R. Ieguminosarum 3855, and Bradyrhizobium sp. BR7301, respectively. A Mn-SOD was present in Rhizobium and two in Bradyrhizobium and bacteroids. Nodule mitochondria from all three legume species had a single Mn-SOD with similar relative mobility, whereas the cytosol contained several CuZn-SODs: two in Phaseolus and Pisum, and four in Vigna. In the cytoplasm of V. unguiculata nodules, a Fe-containing SOD was also present, with an electrophoretic mobility between those of CuZn- and Mn-SODs, and an estimated molecular weight of 57,000. Total SOD activity of the soluble fraction of host cells, expressed on a nodule fresh weight basis, exceeded markedly that of bacteroids. Likewise, specific SOD activities of free-living bacteria were superior or equal to those of their symbiotic forms. Soluble extracts of bacteria and bacteroids did not show peroxidase activity (EC 1.11.1.7), but the nodule cell cytoplasm contained diverse peroxidase isozymes which were readily distinguishable from leghemoglobin components by electrophoresis. Data indicated that peroxidases and leghemoglobins did not significantly interfere with SOD localization on gels. Treatment with chloroform-ethanol scarcely affected the isozymic pattern of SODs and peroxidases, and had limited success in the removal of leghemoglobin.
机译：测定了菜豆，小豌豆和un豆的结节中超氧化物歧化酶（SOD； EC 1.15.1.1）的活性和同工酶组成。由根瘤菌菜豆3622，R。Ieguminosarum 3855和Bradyrhizobium sp。分别为BR7301。根瘤菌中存在Mn-SOD，而根瘤菌和类细菌中存在两种。来自所有三个豆科物种的根瘤线粒体具有单一的Mn-SOD，具有相对的相对迁移率，而胞质溶胶中包含多个CuZn-SOD：两个在菜豆和豌豆中，四个在豌豆中。在圆柏结节的细胞质中，还存在一种含铁的SOD，其电泳迁移率介于CuZn和Mn-SOD的迁移率之间，估计分子量为57,000。以结节新鲜重量为基础表达的宿主细胞可溶性部分的总SOD活性明显超过了类细菌。同样，自由生存细菌的特定SOD活性优于或等于其共生形式。细菌和类细菌的可溶性提取物未显示过氧化物酶活性（EC 1.11.1.7），但结节细胞胞质中含有多种过氧化物酶同工酶，可通过电泳轻易地将其与豆血红蛋白成分区分开。数据表明过氧化物酶和豆球蛋白不会显着干扰凝胶中的SOD定位。用氯仿-乙醇处理几乎不会影响SOD和过氧化物酶的同功酶模式，并且在去除豆血红蛋白方面的成功有限。

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