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首页> 美国卫生研究院文献>Plant Physiology >Electrolyte Leakage Lipoxygenase and Lipid Peroxidation Induced in Tomato Leaf Tissue by Specific and Nonspecific Elicitors from Cladosporium fulvum
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Electrolyte Leakage Lipoxygenase and Lipid Peroxidation Induced in Tomato Leaf Tissue by Specific and Nonspecific Elicitors from Cladosporium fulvum

机译：番茄叶枯病菌特异性和非特异性诱导子诱导番茄叶片组织中的电解质泄漏脂氧化酶和脂质过氧化

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摘要

Glycoprotein nonspecific elicitor (NSE) and a specific elicitor preparation from intercellular fluids (SE) of tomato (Lycopersicon esculentum Mill. cv Bonny Best or Potentate) infected with race 2.4.5 of Cladosporium fulvum Cooke [syn. Fulvia fulva (Cooke) Ciferri] were injected into cv Sonatine (resistant to race 2.4.5) to compare electrolyte leakage, lipoxygenase activity, and lipid peroxidation induced in response to these elicitors. Increased electrolyte leakage was induced by NSE or SE; the leakage due to NSE but not to SE was inhibited by the nonsteroidal antiinflammatory drug (NSAID) piroxicam. Under normal photoperiod conditions, higher levels of lipoxygenase activity were detected 6 hours after injection with either elicitor. This activity peaked by 12 hours with both elicitors and declined to control levels by 24 hours when visible necrosis could be detected. Both NSE and SE-induced lipoxygenase was inhibited by piroxicam in vitro. Lipid peroxidation in elicitor-treated tissue was also assayed at 6, 12, and 24 hours after injection using the TBA test for malonaldehyde. Increased peroxidation was detected in response to NSE or SE at 12 hours with similar values obtained at 24 hours. With plants incubated in the dark, lipoxygenase, and lipid peroxidation were similarly induced in SE-injected tissue whereas necrosis induction by SE was light dependent.
机译：糖蛋白非特异性激发子（NSE）和由番茄（番茄）（Lycopersicon esculentum Mill。cv Bonny Best或Potentate）的细胞间液（SE）感染了Cladosporium fulvum Cooke的种族2.4.5的特异性激发子制剂。将富叶富钴红（Cooke）Ciferri]注入cv Sonatine（对2.4.5小种有抗性），以比较电解质泄漏，脂加氧酶活性和对这些激发子的诱导诱导的脂质过氧化。 NSE或SE引起电解液泄漏增加；非甾体类抗炎药（NSAID）吡罗昔康抑制了NSE而非SE引起的渗漏。在正常的光周期条件下，注射任何一种激发子后6小时都检测到较高水平的脂氧合酶活性。两种引发剂的这种活性均在12小时达到峰值，到24小时，当可以检测到可见的坏死时，活性下降至对照水平。吡罗昔康在体外抑制NSE和SE诱导的脂氧合酶。还使用TBA测试丙二醛在注射后6、12和24小时测定了经激发子处理的组织中的脂质过氧化。在12小时对NSE或SE的反应中检测到过氧化增加，在24小时获得了相似的值。用在黑暗中孵育的植物，在SE注入的组织中类似地诱导脂氧合酶和脂质过氧化，而SE诱导的坏死是光依赖性的。

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