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首页> 美国卫生研究院文献>Plant Physiology >31P-Nuclear Magnetic Resonance Determination of Phosphate Compartmentation in Leaves of Reproductive Soybeans (Glycine max L.) as Affected by Phosphate Nutrition
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31P-Nuclear Magnetic Resonance Determination of Phosphate Compartmentation in Leaves of Reproductive Soybeans (Glycine max L.) as Affected by Phosphate Nutrition

机译：31P-核磁共振法测定受营养影响的生殖大豆叶片中的磷酸根

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摘要

Most leaf phosphorus is remobilized to the seed during reproductive development in soybean. We determined, using 31P-NMR, the effect phosphorus remobilization has on vacuolar inorganic phosphate pool size in soybean (Glycine max [L.] Merr.) leaves with respect to phosphorus nutrition and plant development. Phosphate compartmentation between cytoplasmic and vacuolar pools was observed and followed in intact tissue grown hydroponically, at the R2, R4, and R6 growth stages. As phosphorus in the nutrient solution decreased from 0.45 to 0.05 millimolar, the vacuolar phosphate peak became less prominent relative to cytoplasmic phosphate and hexose monophosphate peaks. At a nutrient phosphate concentration of 0.05 millimolar, the vacuolar phosphate peak was not detectable. At higher levels of nutrient phosphate, as plants progressed from the R2 to the R6 growth stage, the vacuolar phosphate peak was the first to disappear, suggesting that storage phosphate was remobilized to a greater extent than metabolic phosphate. Under suboptimal phosphate nutrition (≤ 0.20 millimolar), the hexose monophosphate and cytoplasmic phosphate peaks declined earlier in reproductive development than when phosphate was present in optimal amounts. Under low phosphate concentrations (0.05 millimolar) cytoplasmic phosphate was greatly reduced. Carbon metabolism was coincidently disrupted under low phosphate nutrition as shown by the appearance of large, prominent starch grains in the leaves. Cytoplasmic phosphate, and leaf carbon metabolism dependent on it, are buffered by vacuolar phosphate until late stages of reproductive growth.
机译：在大豆的生殖发育过程中，大多数叶磷被转移到种子上。我们使用 31 P-NMR确定了磷固着对大豆叶片中液泡状无机磷酸盐池大小的影响，涉及磷营养和植物发育。在R2，R4和R6生长阶段，观察到了细胞质和液泡池之间的磷酸盐区隔，然后在水培生长的完整组织中进行了追踪。当营养液中的磷从0.45摩尔减少到0.05毫摩尔时，液泡状磷酸盐峰相对于胞质磷酸盐和己糖单磷酸盐峰变得不那么突出。在0.05毫摩尔的营养磷酸盐浓度下，无法检测到液泡的磷酸盐峰。在较高水平的营养磷酸盐下，随着植物从R2进入R6的生长阶段，液泡磷酸峰最先消失，这表明贮藏磷酸的迁移程度要高于代谢磷酸。在磷营养不足（≤0.20毫摩尔）的情况下，生殖发育中的己糖一磷酸和细胞质磷酸盐峰值比磷酸盐的最佳含量更早下降。在低磷酸盐浓度（0.05毫摩尔）下，细胞质磷酸盐大大减少。在低磷酸盐营养下，碳代谢同时发生中断，如叶片中出现明显的大淀粉粒所显示。液泡磷酸盐缓冲细胞质磷酸盐和依赖其的叶片碳代谢，直到生殖生长的后期。

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