首页> 美国卫生研究院文献>Plant Physiology >Assembly of the Light-Harvesting Complexes (LHCs) of Photosystem II (Monomeric LHC IIb Complexes Are Intermediates in the Formation of Oligomeric LHC IIb Complexes).
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Assembly of the Light-Harvesting Complexes (LHCs) of Photosystem II (Monomeric LHC IIb Complexes Are Intermediates in the Formation of Oligomeric LHC IIb Complexes).

机译:光系统II的光收集络合物(LHC)的组装(单体LHC IIb络合物是低聚LHC IIb络合物形成的中间体)。

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摘要

The light-induced assembly of light-harvesting complex (LHC) II has been followed during the biogenesis of the plastid. Seedlings grown in intermittent light (IML) accumulate only small amounts of chlorophyll b. The minor LHC II apoproteins are present; however, the apoprotein levels of the major LHC II complex, LHC IIb, are severely depressed after exposure to IML. The levels of all LHC II apoproteins increase rapidly upon exposure to continuous illumination. The 25-kD, type 3 LHC IIb subunit appears to be more abundant during the early hours of greening in relation to its level in mature thylakoids. The LHC IIb apoproteins are initially associated with pigments to form monomeric pigment-protein complexes. The abundance of monomeric LHC IIb complexes gradually decreases during exposure to continuous light and a concomitant increase occurs in the amount of the trimeric and higher-order oligomeric forms. Pulse-chase experiments verify that labeled LHC IIb monomeric complexes are intermediates in the formation of trimeric and higher-order oligomeric LHC IIb-pigmented complexes. Therefore, the assembly of LHC II occurs via the initial pigmentation of the apoproteins to form monomeric complexes and proceeds in a sequential manner.
机译:在质体的生物发生过程中遵循了光诱导的集光复合体(LHC)II的组装。在间歇光照(IML)下生长的幼苗仅积累少量的叶绿素b。存在次要的LHC II载脂蛋白。但是,主要的LHC II复合体LHC IIb的载脂蛋白水平在接触IML后严重降低。暴露于连续光照下,所有LHC II载脂蛋白的水平都会迅速增加。 25 kD,3型LHC IIb亚基在绿化的早期相对于成熟类囊体中的水平似乎更为丰富。 LHC IIb载脂蛋白最初与色素结合形成单体色素-蛋白质复合物。单体LHC IIb复合物的丰度在暴露于连续光的过程中逐渐降低,并且三聚体和高级低聚体形式的数量随之增加。脉冲追踪实验验证了标记的LHC IIb单体复合物是三聚体和高阶低聚LHC IIb色素复合物形成的中间体。因此,LHC II的组装是通过脱辅基蛋白的初始色素沉淀形成的,形成单体复合物,并以顺序方式进行。

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