首页> 美国卫生研究院文献>Plant Physiology >The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis.

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The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis.

机译：Synechococcus sp。中的rbcLS基因组区域。 PCC 7942包含与在低CO2浓度下和叶绿素生物合成中的生长能力有关的基因。

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摘要

Several genes involved in the ability of Synechococcus sp. PCC 7942 to grow under different CO2 concentrations were mapped in the genomic region of rbcLS (the operon encoding the large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase). Insertion of a cartridge encoding kanamycin resistance within open reading frame (ORF) 78, designated ccmJ, located 7 kb upstream of rbcLS, resulted in a kanamycin-resistant, high-CO2-requiring mutant, M3, which does not contain normal carboxysomes. ccmJ shows significant homology to csoS1 encoding a carboxysomal shell polypeptide in Thiobacillus neopolitanus. Analysis of the polypeptide pattern of a carboxysome-enriched fraction indicated several differences between the wild type and the mutant. The amount of the ribulose-1,5-bisphosphate carboxylase/oxygenase subunits was considerably smaller in the carboxysomal fraction of the mutant when compared to the wild type. On the basis of the sequence analyses, ORF286 and ORF466, located downstream of ccmJ, were identified as chlL and chlN, respectively, which are involved in chlorophyll biosynthesis in the dark.

机译：几个基因参与Synechococcus sp。的能力。将在不同CO2浓度下生长的PCC 7942定位在rbcLS的基因组区域（编码核糖-1,5-双磷酸羧化酶/加氧酶的大亚基和小亚基的操纵子）中。在rbcLS上游7 kb的开放阅读框（ORF）78（称为ccmJ）中插入编码卡那霉素抗性的药筒，产生了抗卡那霉素，高CO2需求的突变体M3，该突变体不包含正常的羧基体。 ccmJ与新城硫杆菌中的编码羧化壳多肽的csoS1显示出显着的同源性。对富含羧基体的级分的多肽模式的分析表明，野生型和突变型之间存在一些差异。与野生型相比，在突变体的羧基体级分中核糖-1,5-双磷酸羧化酶/加氧酶亚基的数量要少得多。根据序列分析，位于ccmJ下游的ORF286和ORF466分别被鉴定为chlL和chlN，它们在黑暗中参与叶绿素的生物合成。

著录项

期刊名称 Plant Physiology
作者
M Ronen-Tarazi; J Lieman-Hurwitz; C Gabay; M I Orus; A Kaplan;
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作者单位

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年(卷),期 1995(108),4
年度 1995
页码 1461–1469
总页数 9
原文格式 PDF
正文语种
中图分类人体生理学;
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专利

1. 蓝藻Synechococcus sp.PCC 7942穿梭表达质粒的构建及胸腺素α1基因的表达 [J] . 章军, 秦燕, 欧阳青, 分子细胞生物学报（英文版） . 2003,第001期
2. THE GENOMIC REGION OF RBCLS IN SYNECHOCOCCUS SP PCC 7942 CONTAINS GENES INVOLVED IN THE ABILITY TO CROW UNDER LOW CO2 CONCENTRATION AND IN CHLOROPHYLL BIOSYNTHESIS [J] . Ronentarazi M., Gabay C., Orus MI., Plant physiology . 1995,第4期

机译：SYNECHOCOCCUS SP PCC 7942中RBCLS的基因组区域包含在低CO2浓度下和能够进行叶绿素生物合成的乌鸦所涉及的基因。
3. Modification of topA in Synechococcus sp. PCC 7942 resulted in mutants capable of growing under low but not high concentration of CO2 [J] . Gabay C., Hassidim M., Ronen-Tarazi M., FEMS Microbiology Letters . 1998,第2期

机译：Synechococcus sp。中topA的修饰。 PCC 7942导致突变体能够在低浓度但不是高浓度的CO2下生长
4. Novel gene products associated with NdhD3/D4-containing NDH-1 complexes are involved in photosynthetic CO2 hydration in the cyanobacterium, Synechococcus sp. PCC7942. [J] . Maeda S, Badger MR, Price GD Molecular Microbiology . 2002,第2期

机译：与含NdhD3 / D4的NDH-1复合物相关的新型基因产物参与了蓝细菌Synechococcus sp。的光合作用CO2水合。 PCC7942。
5. Moderate UVB triggers dynamic regulation of cpc, psbA and rbcL transcripts in the cyanobacterium Synechococcus sp. PCC 7942 [C] . DA Campbell, TM MacDonald, LA Dubois, International Congress on Photosynthesis . 2001

机译：中等UVB触发CPC，PSBA和RBCL转录物的动态调节，在肌腱杆菌SP中。 PCC 7942.
6. Gene expression in two cyanobacteria, freshwater Synechococcus sp. PCC 7942 and oceanic Synechococcus sp. WH 7803, in response to ammonium, nitrate or iron. [D] . Sadeghi, Abbas. 1998

机译：在两个蓝藻淡水Synechococcus sp。中的基因表达。 PCC 7942和海洋Synechococcus sp。 WH 7803，响应于铵，硝酸盐或铁。
7. Analysis of a genomic DNA region from the cyanobacterium Synechococcus sp. strain PCC7942 involved in carboxysome assembly and function. [O] . G D Price, S M Howitt, K Harrison, 1993

机译：蓝藻Syechococcus sp。的基因组DNA区域的分析。菌株PCC7942参与了羧基体的组装和功能。
8. The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis. [O] . Ronen-Tarazi, M, Lieman-Hurwitz, J, Gabay, C, 1995

机译：Synechococcus sp。中的rbcLS基因组区域。 PCC 7942包含与在低CO2浓度下和叶绿素生物合成中的生长能力有关的基因。

The genomic region of rbcLS in Synechococcus sp. PCC 7942 contains genes involved in the ability to grow under low CO2 concentration and in chlorophyll biosynthesis.

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