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首页> 美国卫生研究院文献>Plant Physiology >Study of lignification by noninvasive techniques in growing maize internodes. An investigation by Fourier transform infrared cross-polarization-magic angle spinning 13C-nuclear magnetic resonance spectroscopy and immunocytochemical transmission electron microscopy.
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Study of lignification by noninvasive techniques in growing maize internodes. An investigation by Fourier transform infrared cross-polarization-magic angle spinning 13C-nuclear magnetic resonance spectroscopy and immunocytochemical transmission electron microscopy.

机译：利用玉米生长节间无创技术进行木质化的研究。通过傅立叶变换红外交叉极化-魔角旋转13C-核磁共振波谱和免疫细胞化学透射电子显微镜进行研究。

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Noninvasive techniques were used for the study in situ of lignification in the maturing cell walls of the maize (Zea mays L.) stem. Within the longitudinal axis of a developing internode all of the stages of lignification can be found. The synthesis of the three types of lignins, p-hydroxyphenylpropane (H), guaiacyl (G), and syringyl (S), was investigated in situ by cross-polarization-magic angle spinning 13C-solid-state nuclear magnetic resonance, Fourier transform infrared spectroscopy, and immunocytochemical electron microscopy. The first lignin appearing in the parenchyma is of the G-type preceeding the incorporation of S nuclei in the later stages. However, in vascular bundles, typical absorption bands of S nuclei are visible in the Fourier transform infrared spectra at the earliest stage of lignification. Immunocytochemical determination of the three types of lignin in transmission electron microscopy was possible thanks to the use of antisera prepared against synthetic H, G, and the mixed GS dehydrogenative polymers (K. Ruel, O. Faix, J.P. Joseleau [1994] J Trace Microprobe Tech 12: 247-265). The specificity of the immunological probes demonstrated that there are differences in the relative temporal synthesis of the H, G, and GS lignins in the different tissues undergoing lignification. Considering the intermonomeric linkages predominating in the antigens used for the preparation of the immunological probes, the relative intensities of the labeling obtained provided, for the first time to our knowledge, information about the macromolecular nature of lignins (condensed versus noncondensed) in relation to their ultrastructural localization and development stage.
机译：使用非侵入性技术对玉米茎（Zea mays L.）成熟细胞壁中的木质化进行原位研究。在发育中的节间的纵轴内，可以发现木质化的所有阶段。通过交叉极化-磁角旋转13C-固态核磁共振，傅里叶变换原位研究了三种类型的木质素对羟基苯丙烷（H），愈创木脂（G）和丁香基（S）的合成。红外光谱和免疫细胞化学电子显微镜。出现在薄壁组织中的第一个木质素是G型，先于后期加入S核。然而，在维管束中，木质化的最早阶段，傅立叶变换红外光谱中可见S核的典型吸收带。由于使用了针对合成H，G和混合GS脱氢聚合物制备的抗血清，因此可以在透射电子显微镜中对三种类型的木质素进行免疫细胞化学测定（K. Ruel，O. Faix，JP Joseleau [1994] J Trace Microprobe科技12：247-265）。免疫探针的特异性表明，在经历木质化作用的不同组织中，H，G和GS木质素的相对时间合成存在差异。考虑到用于制备免疫探针的抗原中主要的单体间键合，获得的标记的相对强度首次为我们提供了有关木质素（缩合或不缩合）的大分子性质的信息。超微结构的定位和发展阶段。

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