• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Plant Physiology >Involvement of protein kinase and extraplastidic serine/threonine protein phosphatases in signaling pathways regulating plastid transcription and the psbD blue light-responsive promoter in barley.
【2h】

Involvement of protein kinase and extraplastidic serine/threonine protein phosphatases in signaling pathways regulating plastid transcription and the psbD blue light-responsive promoter in barley.

机译：大麦中蛋白激酶和质体外丝氨酸/苏氨酸蛋白磷酸酶参与调控质体转录和psbD蓝光响应性启动子的信号传导途径。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated the signaling pathways that control changes in plastid transcription in response to development and light. Plastid gene expression was analyzed in dark-grown barley (Hordeum vulgare L.) seedlings treated in vivo with an inhibitor of protein phosphatases 1 and 2A, okadaic acid (OA), or an inhibitor of protein kinases (K252a), followed by exposure of the seedlings to either red, blue, or white light. OA prevented blue light from activating the plastid pshD blue-light-responsive promoter (BLRP) and prevented red and blue light from activating the expression of the plastid-encoded rbcl and psbA and the nuclear-encoded RbcS and Lhcb genes. OA reduced total plastid transcription activity in dark- and light-grown seedlings by 77 to 80%, indicating that OA prevented light-responsive transcription by reducing total plastid transcription. In contrast, K252a activated the accumulation of mRNAs arising from the BLRP. Blue light in combination with K252a increased psbD mRNA levels in an additive manner. The results indicate that protein phosphatases 1 and/or 2A, which reside external to the organelle, are required for proper function of plastid transcription and chloroplast development, whereas a protein kinase represses the BLRP in plants grown in the dark.
机译：我们研究了响应发育和光照控制质体转录变化的信号传导途径。在体内用蛋白磷酸酶1和2A抑制剂，冈田酸（OA）或蛋白激酶抑制剂（K252a）处理的大麦（Hordeum vulgare L.）幼苗中分析质体基因表达幼苗发出红色，蓝色或白色的光。 OA阻止了蓝光激活质体pshD蓝光响应启动子（BLRP），并阻止了红光和蓝光激活质体编码的rbcl和psbA以及核编码的RbcS和Lhcb基因的表达。 OA使深色和浅色幼苗中的总类质体转录活性降低77％至80％，这表明OA通过减少总类质体转录阻止了光响应性转录。相反，K252a激活了BLRP产生的mRNA的积累。蓝光与K252a组合以累加方式增加了psbD mRNA水平。结果表明，驻留在细胞器外部的蛋白磷酸酶1和/或2A是质体转录和叶绿体发育的正常功能所必需的，而蛋白激酶可抑制在黑暗中生长的植物中的BLRP。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号