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首页> 美国卫生研究院文献>Plant Physiology >Decrease in Phosphoribulokinase Activity by Antisense RNA in Transgenic Tobacco. Relationship between Photosynthesis Growth and Allocation at Different Nitrogen Levels
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Decrease in Phosphoribulokinase Activity by Antisense RNA in Transgenic Tobacco. Relationship between Photosynthesis Growth and Allocation at Different Nitrogen Levels

机译：反义RNA降低磷酸激酶活性。 转基因烟草。光合作用生长和代谢之间的关系 不同氮水平下的分配

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摘要

To study the direct effects of photosynthesis on allocation of biomass by altering photosynthesis without altering leaf N or nitrate content, phosphoribulokinase (PRK) activity was decreased in transgenic tobacco (Nicotiana tabacum L.) with an inverted tobacco PRK cDNA and plants were grown at different N levels (0.4 and 5 mm NH4NO3). The activation state of PRK increased as the amount of enzyme was decreased genetically at both levels of N. At high N a 94% decrease in PRK activity had only a small effect (20%) on photosynthesis and growth. At low N a 94% decrease in PRK activity had a greater effect on leaf photosynthesis (decreased by up to 50%) and whole-plant photosynthesis (decreased by up to 35%) than at high N. These plants were up to 35% smaller than plants with higher PRK activities because they had less structural dry matter and less starch, which was decreased by 3- to 4-fold, but still accumulated to 24% to 31% of dry weight; young leaves contained more starch than older leaves in older plants. Leaves had a higher ion and water content, and specific leaf area was higher, but allocation between shoot and root was unaltered. In conclusion, low N in addition to a 94% decrease in PRK by antisense reduces the activity of PRK sufficient to diminish photosynthesis, which limits biomass production under conditions normally considered sink limited.
机译：为了研究通过改变光合作用而不改变叶氮或硝酸盐含量而对光合作用对生物量分配的直接影响，在带有反向烟草PRK cDNA的转基因烟草（Nicotiana tabacum L.）中降低了磷酸核糖激酶（PRK）活性，并在不同的植物上生长N含量（0.4和5毫米NH4NO3）。在两种氮水平下，PRK的活化状态均随着酶量的减少而增加。在高氮水平下，PRK活性下降94％对光合作用和生长的影响很小（20％）。在低氮下，PRK活性降低94％对叶片光合作用（最多降低50％）和整株光合作用（最多降低35％）的影响大于高氮。这些植物最多可提高35％。比具有较高PRK活性的植物小，因为它们的结构干物质少，淀粉少，淀粉减少了3至4倍，但仍积累到干重的24％至31％。在老龄植物中，幼叶比老叶含有更多的淀粉。叶子的离子和水含量更高 含量和比叶面积较高，但之间的分配 芽和根未改变。总之，除了 反义使PRK降低94％，降低了PRK的活性 足以减少光合作用，从而限制了生物量的产生 在通常认为水槽有限的条件下。

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