• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Plant Physiology >Ectopic Expression of Carpel-Specific MADS Box Genes from Lily and Lisianthus Causes Similar Homeotic Conversion of Sepal and Petal in Arabidopsis
【2h】

Ectopic Expression of Carpel-Specific MADS Box Genes from Lily and Lisianthus Causes Similar Homeotic Conversion of Sepal and Petal in Arabidopsis

机译：百合和桔梗心皮特异的MADS盒基因的异位表达导致拟南芥的萼片和花瓣类似的同态转化

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two MADS box genes, Lily MADS Box Gene 2 (LMADS2) and Eustoma grandiflorum MADS Box Gene 1 (EgMADS1), with an extensive similarity to the petunia (Petunia hybrida) FLORAL BINDING PROTEIN 7/11 and Arabidopsis AGL11, were characterized from the lily (Lilium longiflorum) and lisianthus (Eustoma grandiflorum). The expression of LMADS2 and EgMADS1 mRNA was restricted to the carpel and was absent in the other flower organs or vegetative leaves. LMADS2 mRNA was detected mainly in ovules and weakly in style tissues of the carpel, whereas EgMADS1 mRNA was only expressed in the ovules. Transgenic Arabidopsis plants ectopically expressing LMADS2 or EgMADS1 showed similar novel phenotypes resembling 35S::AGAMOUS plants by significantly reducing plant size, flowering early, and losing inflorescence indeterminacy. Ectopic expression of these two genes also generated similar ap2-like flowers by inducing homeotic conversion of the sepals into carpel-like structures in which stigmatic papillae and ovules were observed. In addition, the petals were converted into stamen-like structures in the second whorl of 35S::LMADS2 and 35S::EgMADS1 transgenic Arabidopsis. Our data indicated that LMADS2 and EgMADS1 are putative D functional MADS box genes in lily and lisianthus with a function similar to C functional genes once ectopically expressed in Arabidopsis.
机译：从百合中鉴定了两个MADS盒基因，百合MADS盒基因2（LMADS2）和桔梗MADS盒基因1（EgMADS1），与矮牵牛（Petunia hybrida）FLORAL BINDING PROTEIN 7/11和拟南芥AGL11具有广泛相似性。 （Lilium longiflorum）和桔梗（Eustoma grandiflorum）。 LMADS2和EgMADS1 mRNA的表达仅限于心皮，而在其他花器官或营养叶中则不存在。 LMADS2 mRNA主要在胚珠的胚珠中被检测到，而在心房的体征组织中则较弱，而EgMADS1 mRNA仅在胚珠中表达。异位表达LMADS2或EgMADS1的转基因拟南芥植物表现出相似的新表型，类似于 35S :: AGAMOUS 植物，通过显着减小植物大小，早期开花并失去花序不确定性。这两个基因的异位表达还通过诱导萼片向心皮状结构的顺同性转化而产生相似的 ap2 样花，其中观察到有柱头状乳头和胚珠。此外，在转基因35S :: LMADS2 和 35S :: EgMADS1 拟南芥的第二轮中，花瓣转化为雄蕊状结构。我们的数据表明 LMADS2 和 EgMADS1 是百合和桔梗中推定的D功能MADS盒基因，其功能类似于曾经在拟南芥中异位表达的C功能基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号