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Spatial and Temporal Effects of Free-Air CO2 Enrichment (POPFACE) on Leaf Growth Cell Expansion and Cell Production in a Closed Canopy of Poplar

机译:在密闭的杨树冠层中空气中CO2浓度升高(POPFACE)对叶片生长细胞膨胀和细胞产生的时空影响

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摘要

Leaf expansion in the fast-growing tree, Populus × euramericana was stimulated by elevated [CO2] in a closed-canopy forest plantation, exposed using a free air CO2 enrichment technique enabling long-term experimentation in field conditions. The effects of elevated [CO2] over time were characterized and related to the leaf plastochron index (LPI), and showed that leaf expansion was stimulated at very early (LPI, 0–3) and late (LPI, 6–8) stages in development. Early and late effects of elevated [CO2] were largely the result of increased cell expansion and increased cell production, respectively. Spatial effects of elevated [CO2] were also marked and increased final leaf size resulted from an effect on leaf area, but not leaf length, demonstrating changed leaf shape in response to [CO2]. Leaves exhibited a basipetal gradient of leaf development, investigated by defining seven interveinal areas, with growth ceasing first at the leaf tip. Interestingly, and in contrast to other reports, no spatial differences in epidermal cell size were apparent across the lamina, whereas a clear basipetal gradient in cell production rate was found. These data suggest that the rate and timing of cell production was more important in determining leaf shape, given the constant cell size across the leaf lamina. The effect of elevated [CO2] imposed on this developmental gradient suggested that leaf cell production continued longer in elevated [CO2] and that basal increases in cell production rate were also more important than altered cell expansion for increased final leaf size and altered leaf shape in elevated [CO2].
机译:在密闭林冠人工林中,高浓度的[CO2]刺激了速生树Populus×euramericana的叶片膨胀,并使用自由空气CO2富集技术进行暴露,从而可以在田间条件下进行长期试验。表征[CO2]随时间升高的影响,并与叶片塑性同步指数(LPI)相关,并显示在早期(LPI,0–3)和晚期(LPI,6–8)阶段刺激叶片膨胀。发展。 [CO2]升高的早期和晚期效应分别主要是细胞膨胀增加和细胞产量增加的结果。还显着提高了[CO2]的空间效应,最终叶尺寸的增加是由对叶面积的影响引起的,但对叶长没有影响,这表明对[CO2]的响应使叶片形状发生了变化。通过显示七个脉络区域进行研究,叶片表现出基叶状的叶片发育梯度,首先在叶尖停止生长。有趣的是,与其他报道相反,整个薄片的表皮细胞大小没有明显的空间差异,而细胞生成速率却有明显的基底梯度。这些数据表明,考虑到整个叶片的恒定细胞大小,在决定叶片形状时细胞产生的速率和时间更为重要。 [CO2]升高对这种发育梯度的影响表明,[CO2]升高时,叶片细胞的生产持续时间更长,并且基本的细胞生产率的提高也比改变细胞膨胀对于增加最终叶片尺寸和改变叶片形状更为重要。升高的[CO2]。

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