• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Plant Physiology >The NADPH-Dependent Thioredoxin Reductase/Thioredoxin System in Germinating Barley Seeds: Gene Expression Protein Profiles and Interactions between Isoforms of Thioredoxin h and Thioredoxin Reductase
【2h】

The NADPH-Dependent Thioredoxin Reductase/Thioredoxin System in Germinating Barley Seeds: Gene Expression Protein Profiles and Interactions between Isoforms of Thioredoxin h and Thioredoxin Reductase

机译：NADPH依赖的硫氧还蛋白还原酶/硫氧还蛋白系统在发芽的大麦种子中：基因表达蛋白质概况以及硫氧还蛋白h和硫氧还蛋白还原酶同工型之间的相互作用

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The NADPH-dependent thioredoxin reductase (NTR)/thioredoxin (Trx) system catalyzes disulfide bond reduction in the cytoplasm and mitochondrion. Trx h is suggested to play an important role in seed development, germination, and seedling growth. Plants have multiple isoforms of Trx h and NTR; however, little is known about the roles of the individual isoforms. Trx h isoforms from barley (Hordeum vulgare) seeds (HvTrxh1 and HvTrxh2) were characterized previously. In this study, two NTR isoforms (HvNTR1 and HvNTR2) were identified, enabling comparison of gene expression, protein appearance, and interaction between individual NTR and Trx h isoforms in barley embryo and aleurone layers. Although mRNA encoding both Trx h isoforms is present in embryo and aleurone layers, the corresponding proteins differed in spatiotemporal appearance. HvNTR2, but not HvNTR1, gene expression seems to be regulated by gibberellic acid. Recombinant HvNTR1 and HvNTR2 exhibited virtually the same affinity toward HvTrxh1 and HvTrxh2, whereas HvNTR2 has slightly higher catalytic activity than HvNTR1 with both Trx h isoforms, and HvNTR1 has slightly higher catalytic activity toward HvTrxh1 than HvTrxh2. Notably, both NTRs reduced Trx h at the acidic conditions residing in the starchy endosperm during germination. Interspecies reactions between the barley proteins and Escherichia coli Trx or Arabidopsis thaliana NTR, respectively, occurred with 20- to 90-fold weaker affinity. This first investigation of regulation and interactions between members of the NTR/Trx system in barley seed tissues suggests that different isoforms are differentially regulated but may have overlapping roles, with HvNTR2 and HvTrxh1 being the predominant isoforms in the aleurone layer.
机译：NADPH依赖的硫氧还蛋白还原酶（NTR）/硫氧还蛋白（Trx）系统催化细胞质和线粒体中的二硫键还原。建议Trx h在种子发育，发芽和幼苗生长中起重要作用。植物具有Trx h和NTR的多种同工型；然而，关于单个同工型的作用知之甚少。大麦（Hordeum vulgare）种子（HvTrxh1和HvTrxh2）的Trx h亚型已经过表征。在这项研究中，确定了两种NTR亚型（HvNTR1和HvNTR2），从而能够比较基因表达，蛋白质外观以及大麦胚和糊粉层中各个NTR和Trx h亚型之间的相互作用。尽管在胚胎和糊粉层中都存在编码两种Trx h亚型的mRNA，但相应的蛋白质在时空上却有所不同。 HvNTR2，而不是HvNTR1，基因表达似乎受赤霉素调节。重组HvNTR1和HvNTR2对HvTrxh1和HvTrxh2表现出几乎相同的亲和力，而HvNTR2的催化活性略高于同时具有Trx h同工型的HvNTR1，HvNTR1的HvTrxh1催化活性比HvTrxh2略高。值得注意的是，两种NTR在发芽过程中在淀粉质胚乳中的酸性条件下均会降低Trx h。大麦蛋白与大肠杆菌Trx或拟南芥NTR之间的种间反应分别以弱20到90倍的亲和力发生。对大麦种子组织中NTR / Trx系统成员之间的调节和相互作用的首次研究表明，不同的同工型受到差异调节，但可能具有重叠的作用，其中HvNTR2和HvTrxh1是糊粉层的主要同工型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号