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首页> 美国卫生研究院文献>Plant Physiology >Seed Storage Oil Mobilization Is Important But Not Essential for Germination or Seedling Establishment in Arabidopsis
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Seed Storage Oil Mobilization Is Important But Not Essential for Germination or Seedling Establishment in Arabidopsis

机译：种子贮藏油的动员对于拟南芥的发芽或育苗至关重要但不是必需的

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摘要

Triacylglycerol (TAG) is a major storage reserve in many plant seeds. We previously identified a TAG lipase mutant called sugar-dependent1 (sdp1) that is impaired in TAG hydrolysis following Arabidopsis (Arabidopsis thaliana) seed germination (). The aim of this study was to identify additional lipases that account for the residual TAG hydrolysis observed in sdp1. Mutants were isolated in three candidate genes (SDP1-LIKE [SDP1L], ADIPOSE TRIGLYCERIDE LIPASE-LIKE, and COMPARATIVE GENE IDENTIFIER-58-LIKE). Analysis of double, triple, and quadruple mutants showed that SDP1L is responsible for virtually all of the residual TAG hydrolysis present in sdp1 seedlings. Oil body membranes purified from sdp1 sdp1L seedlings were deficient in TAG lipase activity but could still hydrolyze di- and monoacylglycerol. SDP1L is expressed less strongly than SDP1 in seedlings. However, SDP1L could partially rescue TAG breakdown in sdp1 seedlings when expressed under the control of the SDP1 or 35S promoters and in vitro assays showed that both SDP1 and SDP1L can hydrolyze TAG, in preference to diacylglycerol or monoacylglycerol. Seed germination was slowed in sdp1 sdp1L and postgerminative seedling growth was severely retarded. The frequency of seedling establishment was also reduced, but sdp1 sdp1L was not seedling lethal under normal laboratory growth conditions. Our data show that together SDP1 and SDP1L account for at least 95% of the rate of TAG hydrolysis in Arabidopsis seeds, and that this hydrolysis is important but not essential for seed germination or seedling establishment.
机译：三酰甘油（TAG）是许多植物种子中的主要储存储备。我们先前确定了一种称为糖依赖性1（sdp1）的TAG脂肪酶突变体，该突变体在拟南芥（Arabidopsis thaliana）种子萌发后的TAG水解中受损。这项研究的目的是确定额外的脂肪酶，以解决sdp1中观察到的残留TAG水解。在三个候选基因（SDP1-LIKE [SDP1L]，ADIPOSE TRIGLYCERIDE LIPase-Like和COMPENETIVE GENE IDENTIFIER-58-LIKE）中分离了突变体。对双突变，三突变和四突变的分析表明，SDP1L几乎负责sdp1幼苗中存在的所有残留TAG水解。从sdp1 sdp1L幼苗纯化的油体膜的TAG脂肪酶活性不足，但仍可以水解二和单酰基甘油。在幼苗中，SDP1L的表达强度不及 SDP1 。然而，当在 SDP1 或 35S 启动子的控制下表达时，SDP1L可以部分挽救 sdp1 幼苗中的TAG降解，体外试验表明， SDP1和SDP1L可以水解TAG，优先于二酰基甘油或单酰基甘油。 sdp1 sdp1L 的种子发芽减慢，发芽后幼苗的生长受到严重阻碍。幼苗建立的频率也降低了，但是 sdp1 sdp1L 在正常实验室生长条件下对幼苗没有致死性。我们的数据表明，SDP1和SDP1L共同占拟南芥种子中TAG水解率的至少95％，并且这种水解作用对种子发芽或幼苗生长很重要，但不是必需的。

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