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首页> 美国卫生研究院文献>Plant Signaling Behavior >Cloning of 9-cis-epoxycarotenoid dioxygenase (NCED) gene and the role of ABA on fruit ripening
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Cloning of 9-cis-epoxycarotenoid dioxygenase (NCED) gene and the role of ABA on fruit ripening

机译：9-顺式-环氧类胡萝卜素双加氧酶（NCED）基因的克隆及其在果实成熟中的作用

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In order to understand more details about the role of abscisic acid (ABA) in fruit ripening and senescence, six 740 bp cDNAs (LeNCED1, LeNCED2, PpNCED1, VVNCED1, DKNCED1 and CMNCED1) which encode 9-cis-epoxycarotenoid dioxygenase (NCED) as a key enzyme in ABA biosynthesis, were cloned from fruits of tomato, peach, grape, persimmon and melon using an RT-PCR approach. A Blast homology search revealed a similarity of amino acid 85.76% between the NCEDs. A relationship between ABA and ethylene during ripening was also investigated. At the mature green stage, exogenous ABA treatment increased ABA content in flesh, and promoting ethylene synthesis and fruit ripening, while treatment with nordihydroguaiaretic acid (NDGA), inhibited them, delayed fruit ripening and softening. However, ABA inhibited the ethylene synthesis obviously while NDGA promoted them when treated the immature fruit with these chemicals. At the breaker, NDGA treatment cannot block ABA accumulation and ethylene synthesis. Based on the results obtained in this study, it was concluded that ABA plays different role in ethylene synthesis system in different stages of tomato fruit ripening.
机译：为了了解有关脱落酸（ABA）在果实成熟和衰老中的作用的更多细节，编码了9个顺式-环氧类胡萝卜素双加氧酶（NCED）的6个740 bp cDNA（LeNCED1，LeNCED2，PpNCED1，VVNCED1，DKNCED1和CMNCED1）。利用RT-PCR方法从番茄，桃子，葡萄，柿子和甜瓜的果实中克隆了ABA生物合成中的关键酶。 Blast同源性搜索显示NCED之间的氨基酸相似度为85.76％。还研究了成熟过程中ABA与乙烯之间的关系。在成熟的绿色阶段，外源ABA处理可增加果肉中ABA含量，并促进乙烯合成和果实成熟，而去甲二氢愈创木酸（NDGA）处理可抑制它们，延迟果实成熟和软化。然而，用这些化学物质处理未成熟果实时，ABA明显抑制乙烯的合成，而NDGA促进了乙烯的合成。在破碎机上，NDGA处理不能阻止ABA的积累和乙烯的合成。根据本研究的结果，可以得出结论，ABA在番茄果实成熟的不同阶段在乙烯合成系统中发挥不同的作用。

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