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首页> 美国卫生研究院文献>Plants >A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
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A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement

机译：四种基因型冬油菜叶片衰老过程中蛋白水解机理的比较研究突出了改善NRE的相关生理和分子特性

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摘要

Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding.
机译：冬季油菜的特点是氮素利用效率低，与营养阶段的叶片氮素迁移效率（NRE）弱有关。通过研究叶片NRE的自然基因型变异性，我们的目标是表征与硝酸盐供应充足或受限相关的有效的蛋白水解作用和高叶片NRE相关的生理特性和主要蛋白酶类别。可溶性蛋白和D1蛋白（类囊体结合蛋白）的降解速率与氮固位相关，除了萨穆拉伊基因型（尽管蛋白水解水平很高）显示出低的NRE。在受限的硝酸盐条件下，在酸性pH下，高水平的可溶性蛋白质降解与丝氨酸，半胱氨酸和天冬氨酸蛋白酶有关。低叶NRE与可溶性蛋白和类囊体结合蛋白的弱蛋白水解作用有关。基因型Samouraï获得的结果表明，蛋白水解开始和脱落之间的时间可能是决定因素。酸性蛋白酶的特定参与表明自噬和/或与衰老相关的液泡与在低氮条件下的氮重新固定有关。数据显示，D1降解速率可能是叶片NRE的相关指标，并且可以用作植物育种的工具。

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