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首页> 美国卫生研究院文献>Journal of Virology >Identification and Characterization of Two Closely Related Unclassifiable Endogenous Retroviruses in Pythons (Python molurus and Python curtus)
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Identification and Characterization of Two Closely Related Unclassifiable Endogenous Retroviruses in Pythons (Python molurus and Python curtus)

机译：在Python中识别和表征两种密切相关的无法分类的内源性逆转录病毒（Python molurus和Python curtus）

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Boid inclusion body disease (BIBD) is a fatal disorder of boid snakes that is suspected to be caused by a retrovirus. In order to identify this agent, leukocyte cultures (established from Python molurus specimens with symptoms of BIBD or kept together with such diseased animals) were assessed for reverse transcriptase (RT) activity. Virus from cultures exhibiting high RT activity was banded on sucrose density gradients, and the RT peak fraction was subjected to highly efficient procedures for the identification of unknown particle-associated retroviral RNA. A 7-kb full retroviral sequence was identified, cloned, and sequenced. This virus contained intact open reading frames (ORFs) for gag, pro, pol, and env, as well as another ORF of unknown function within pol. Phylogenetic analysis showed that the virus is distantly related to viruses from both the B and D types and the mammalian C type but cannot be classified. It is present as a highly expressed endogenous retrovirus in all P. molurus individuals; a closely related, but much less expressed virus was found in all tested Python curtus individuals. All other boid snakes tested, including Python regius, Python reticulatus, Boa constrictor, Eunectes notaeus, and Morelia spilota, were virus negative, independent of whether they had BIBD or not. Virus isolated from P. molurus could not be transmitted to the peripheral blood mononuclear cells of B. constrictor or P. regius. Thus, there is no indication that this novel virus, which we propose to name python endogenous retrovirus (PyERV), is causally linked with BIBD.
机译：Boid包涵体疾病（BIBD）是致命的Boid蛇疾病，怀疑是由逆转录病毒引起的。为了鉴定这种试剂，评估了白细胞培养物（从具有BIBD症状的巨蟒标本中建立或与此类患病动物一起饲养）的逆转录酶（RT）活性。来自具有高RT活性的培养物的病毒在蔗糖密度梯度上结合，并对RT峰馏分进行高效程序鉴定未知的颗粒相关逆转录病毒RNA。鉴定，克隆并测序了7kb的完整逆转录病毒序列。该病毒包含针对gag，pro，pol和env的完整开放阅读框（ORF），以及pol中另一个功能未知的ORF。系统发育分析表明，该病毒与B型和D型以及哺乳动物C型的病毒都具有远缘关系，但无法分类。它以高表达的内源性逆转录病毒存在于所有软体动物中。在所有测试过的Python curtus个人中发现了一种密切相关但表达较少的病毒。所有其他测试的蛇蛇，包括Python regius，Python reticulatus，蟒蛇，Eunectes notaeus和Morelia spilota，均呈病毒阴性，而与是否患有BIBD无关。从小白僵菌中分离出的病毒不能传播到缩窄双歧杆菌或里氏假单胞菌的外周血单核细胞中。因此，没有迹象表明我们提议将其命名为python内源性逆转录病毒（PyERV）的这种新型病毒与BIBD因果相关。

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