• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>PLoS Genetics >The Drosophila eve Insulator Homie Promotes eve Expression and Protects the Adjacent Gene from Repression by Polycomb Spreading
【2h】

The Drosophila eve Insulator Homie Promotes eve Expression and Protects the Adjacent Gene from Repression by Polycomb Spreading

机译：果蝇前夕绝缘子同源促进前夕表达和保护相邻基因免受压制通过聚梳传播。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Insulators can block the action of enhancers on promoters and the spreading of repressive chromatin, as well as facilitating specific enhancer-promoter interactions. However, recent studies have called into question whether the activities ascribed to insulators in model transgene assays actually reflect their functions in the genome. The Drosophila even skipped (eve) gene is a Polycomb (Pc) domain with a Pc-group response element (PRE) at one end, flanked by an insulator, an arrangement also seen in other genes. Here, we show that this insulator has three major functions. It blocks the spreading of the eve Pc domain, preventing repression of the adjacent gene, TER94. It prevents activation of TER94 by eve regulatory DNA. It also facilitates normal eve expression. When Homie is deleted in the context of a large transgene that mimics both eve and TER94 regulation, TER94 is repressed. This repression depends on the eve PRE. Ubiquitous TER94 expression is “replaced” by expression in an eve pattern when Homie is deleted, and this effect is reversed when the PRE is also removed. Repression of TER94 is attributable to spreading of the eve Pc domain into the TER94 locus, accompanied by an increase in histone H3 trimethylation at lysine 27. Other PREs can functionally replace the eve PRE, and other insulators can block PRE-dependent repression in this context. The full activity of the eve promoter is also dependent on Homie, and other insulators can promote normal eve enhancer-promoter communication. Our data suggest that this is not due to preventing promoter competition, but is likely the result of the insulator organizing a chromosomal conformation favorable to normal enhancer-promoter interactions. Thus, insulator activities in a native context include enhancer blocking and enhancer-promoter facilitation, as well as preventing the spread of repressive chromatin.
机译：绝缘子可以阻断增强子对启动子的作用和抑制性染色质的扩散，并促进特定的增强子-启动子相互作用。然而，最近的研究使人们怀疑，在模型转基因试验中归因于绝缘子的活性是否实际上反映了它们在基因组中的功能。果蝇甚至被跳过的基因（eve）是一个Polycomb（Pc）域，一端具有Pc-group响应元件（PRE），侧翼是一个绝缘子，这种排列在其他基因中也可以看到。在这里，我们表明该绝缘子具有三个主要功能。它阻止了eve Pc结构域的扩散，阻止了邻近基因TER94的阻遏。它可以防止前夕调控DNA激活TER94。它还有助于正常的前夕表达。当在模仿eve和TER94调控的大型转基因中删除同源基因时，TER94被抑制。此抑制取决于前夕PRE。删除Homie时，以eve模式将“无处不在的TER94”表达“替换”，如果还删除了PRE，则这种效果会相反。 TER94的抑制归因于eve Pc域扩散到TER94基因座，并伴随着赖氨酸27处组蛋白H3三甲基化的增加。其他PRE可以在功能上替代 eve PRE，而其他绝缘子则可以阻止在这种情况下，PRE依赖性抑制。 eve 启动子的全部活性也取决于Homie，其他绝缘子也可以促进正常的 eve 增强子-启动子通讯。我们的数据表明这不是由于阻止启动子竞争，而是可能是绝缘子组织了有利于正常增强子-启动子相互作用的染色体构象的结果。因此，天然情况下的绝缘子活性包括增强子阻断和促进子-促进子，以及防止抑制性染色质的扩散。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号