首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Requirement for cellular protein synthesis in reversal of ethidium-bormide-induced inhibition of cell transformation by murine sarcoma virus.
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Requirement for cellular protein synthesis in reversal of ethidium-bormide-induced inhibition of cell transformation by murine sarcoma virus.

机译:逆转溴化乙锭诱导的鼠肉瘤病毒对细胞转化的抑制作用对细胞蛋白质合成的要求。

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摘要

Cultures of mouse Balb 3T3 fibroblasts exposed to a noncytotoxic dose of ethidium bromide for 16-18 hr are unable to produce foci after infection with murine sarcoma virus. Such cultures regain susceptibility to infection when incubated for 6-8 hr in drug-free growth medium. Pretreated but not untreated cultures exhibit sensitivity toward brief (6 hr) exposure to cycloheximide, chloramphenicol, and actinomycin D before infection. Pretreatment with cordy-cepin inhibits the ability of cultures to produce foci after infection. The recovery of ethidium-bromide-treated cultures requires the synthesis of cellular proteins which may have some important role in the establishment of RNA tumor virus infection.
机译:暴露于非细胞毒性剂量的溴化乙锭16-18小时的小鼠Balb 3T3成纤维细胞培养物在感染鼠肉瘤病毒后无法产生病灶。当在无药物生长培养基中孵育6-8小时时,此类培养物可恢复对感染的敏感性。预处理但未经处理的培养物对感染前短暂暴露于环己酰亚胺,氯霉素和放线菌素D的敏感性(6小时)。用Cordy-cepin进行预处理可抑制培养物在感染后产生病灶的能力。溴化乙锭处理过的培养物的回收需要细胞蛋白的合成,这可能对建立RNA肿瘤病毒感染具有重要作用。

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