首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Comparison between growth characteristics of an Epstein--Barr virus (EBV)-genome-negative lymphoma line and its EBV-converted subline in vitro.
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Comparison between growth characteristics of an Epstein--Barr virus (EBV)-genome-negative lymphoma line and its EBV-converted subline in vitro.

机译:爱泼斯坦-巴尔病毒(EBV)基因组阴性淋巴瘤细胞系及其EBV转化亚系体外生长特性的比较。

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摘要

The GC-BJAB cell line, which carries the Epstein--Barr virus (EBV), was derived from an EBV-genome-negative lymphoma line (BJAB) by EBV infection in vitro [G. B. Clements, G. Klein, and S. Povey (1975) Int. J. Cancer, in press]. Both lines grow at a similar rate at 37 degrees but they differ at other temperatures. BJAB grows well at 34 degrees, 37 degrees, and 39 degrees. GC-BJAB grows at 37 degrees and 39 degrees, but grows poorly at 34 degrees. At 37 degrees, GC-BJAB cultures can be maintained at the viable state for a long time after having reached saturation density (approximately 10(6) cells per ml). In contrast, BJAB cultures die very soon after having attained similar maximum density. Since the identity of the two cell lines has been critically established [Clements et al.; E. Svedmyr and M. Jondal (1975) Proc. Nat. Acad. Sci USA 72, 1622--1626; G. Klein, to be published; J. Zeuthen, personal communication] the remarkable differences in their growth properties must be attributed to the EBV genome.
机译:携带爱泼斯坦-巴尔病毒(EBV)的GC-BJAB细胞系是通过体外EBV感染从EBV基因组阴性淋巴瘤系(BJAB)衍生而来的[G. B.克莱门茨,G。克莱因和S.波维(1975)国际。 J.巨蟹座,印刷中]。两条线在37度下均以相似的速率生长,但在其他温度下会有所不同。 BJAB在34度,37度和39度时生长良好。 GC-BJAB在37度和39度生长,但在34度时生长较差。在达到37度时,GC-BJAB培养物在达到饱和密度(每毫升约10(6)个细胞)后可以长时间维持在存活状态。相反,BJAB培养物在达到相似的最大密度后很快就会死亡。由于已经确定了两种细胞系的同一性[Clements et al .; E.Svedmyr和M.Jondal(1975),美国国家科学院院刊。纳特学院美国科学(Sci USA)72,1622--1626; G. Klein,即将出版; [J. Zeuthen,个人交流]其生长特性的显着差异必须归因于EBV基因组。

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