首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >A RNA.DNA hybrid that can adopt two conformations: an x-ray diffraction study of poly(rA).poly(dT) in concentrated solution or in fibers.
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A RNA.DNA hybrid that can adopt two conformations: an x-ray diffraction study of poly(rA).poly(dT) in concentrated solution or in fibers.

机译:RNA.DNA杂合体可以采用两种构象:浓溶液或纤维中的poly(rA).poly(dT)的X射线衍射研究。

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摘要

The RNA.DNA hybrid poly(rA).poly(dT) can adopt two conformations, depending upon its degree of hydration. Fibers yield a conventional A' RNA-like pattern at 79% relative humidity. In contrast, under highly solvated conditions this material yields an x-ray diffraction pattern with striking similarities to that of B DNA and clearly different from the RNA-like diffraction patterns previously obtained from fibers of other hybrids. A structural model is proposed for the solution form of the hybrid that has several similarities to models proposed for B DNA. The hybrid model accommodates the 2'-hydroxyl groups of the poly(rA) strand in intrachain hydrogen bonds to the adjacent ribose moieties.
机译:RNA.DNA杂化poly(rA).poly(dT)可以根据其水合程度采用两种构象。纤维在79%的相对湿度下会产生常规的A'RNA样图案。相反,在高度溶剂化的条件下,该材料产生的X射线衍射图谱与B DNA的显着相似,并且明显不同于先前从其他杂种纤维获得的类RNA的衍射图谱。为杂交体的溶液形式提出了结构模型,该结构模型与针对B DNA提出的模型具有若干相似性。杂合模型容纳与相邻核糖部分的链内氢键中的poly(rA)链的2'-羟基。

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