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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Differential subcellular localization of in vivo-phosphorylated and nonphosphorylated middle-sized tumor antigen of polyoma virus and its relationship to middle-sized tumor antigen phosphorylating activity in vitro.
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Differential subcellular localization of in vivo-phosphorylated and nonphosphorylated middle-sized tumor antigen of polyoma virus and its relationship to middle-sized tumor antigen phosphorylating activity in vitro.

机译：多瘤病毒体内磷酸化和非磷酸化中型肿瘤抗原的亚细胞定位差异及其与体外中型肿瘤抗原磷酸化活性的关系。

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摘要

A small fraction of polyoma virus middle-sized tumor (T) antigen is phosphorylated in vivo, resulting in a small amount of phosphotyrosine and phosphothreonine and significantly larger amounts of phosphoserine. When infected cells are separated into nuclear, plasma membrane, and low-speed supernatant fractions, 80-95% of in vivo-phosphorylated middle-sized T antigen is localized to the plasma membrane fraction, while 25-50% of [35S]methionine-labeled middle-sized T antigen is found in the nuclear fraction and the same amount is found in the plasma membrane fraction. Immunoprecipitated T antigens contain a protein kinase activity that phosphorylates middle-sized T antigen at tyrosine residues. Eighty to 90% of this activity is located in the plasma membrane fraction. When immunoprecipitated T antigens are treated with alkaline phosphatase, middle-sized T antigen-phosphorylating activity decreases as 32PO4 is lost from in vivo 32P-labeled middle-sized T antigen. The possibility that in vivo-phosphorylated middle-sized T antigen located in the plasma membrane is an active tyrosine-specific kinase is discussed.
机译：一小部分的多瘤病毒中型肿瘤（T）抗原在体内被磷酸化，从而导致少量的磷酸酪氨酸和磷酸苏氨酸，以及大量的磷酸丝氨酸。当被感染的细胞分为核膜，质膜和低速上清液部分时，体内磷酸化的中等大小的T抗原的80-95％位于质膜部分，而[35S]蛋氨酸的25-50％在核级分中发现了标记的中等大小的T抗原，在质膜级分中发现了相同数量的T抗原。免疫沉淀的T抗原包含一种蛋白激酶活性，该蛋白可在酪氨酸残基处磷酸化中等大小的T抗原。该活性的百分之八十至百分之九十位于质膜部分。当用碱性磷酸酶处理免疫沉淀的T抗原时，由于体内32P标记的中型T抗原丢失了32PO4，中型T抗原的磷酸化活性降低。讨论了位于质膜中的体内磷酸化中型T抗原是一种活性酪氨酸特异性激酶的可能性。

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