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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Measurement of the repulsive force between polyelectrolyte molecules in ionic solution: hydration forces between parallel DNA double helices.
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Measurement of the repulsive force between polyelectrolyte molecules in ionic solution: hydration forces between parallel DNA double helices.

机译：离子溶液中聚电解质分子之间的排斥力的测量：平行DNA双螺旋之间的水合力。

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摘要

We have measured the repulsive force between B-form double helices in parallel packed arrays of polymer-condensed DNA in the presence of 0.005-1.0 M ionic solutions. Molecular repulsion is consistently exponential with a 2.5-3.5 A decay distance, when the separation between DNA surfaces is 5-15 A. Only weakly dependent on ionic strength and independent of molecular size, this intermolecular repulsion does not obey the predictions of electrostatic double-layer theory. Rather, it strongly resembles the "hydration force" first recognized and quantified between phospholipid bilayers. Only beyond 15 A separation between molecules is there evidence of electrostatic double-layer forces. The quantitative failure of electrostatic double-layer theory seen here must gravely affect accepted analyses of other polyelectrolyte systems. Because the packing of condensed DNA resembles the spacings of DNA in many bacteriophages, our results permit estimation of the "DNA pressure" in phage heads.
机译：我们已经测量了在0.005-1.0 M离子溶液存在下，聚合物浓缩DNA的平行堆积阵列中B型双螺旋之间的排斥力。当DNA表面之间的间距为5-15 A时，分子斥力始终以2.5-3.5 A的衰减距离为指数。仅弱依赖于离子强度且与分子大小无关，这种分子间斥力并没有遵循静电双原子的预测。层理论。而是，它非常类似于首先在磷脂双层之间识别和定量的“水合力”。只有分子之间的间隔超过15 A，才有静电双层力的证据。此处看到的静电双层理论的定量失效必须严重影响其他聚电解质系统的公认分析。因为浓缩的DNA的堆积类似于许多噬菌体中的DNA间距，所以我们的结果允许估算噬菌体头部中的“ DNA压力”。

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