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Mutations in the Human Immunodeficiency Virus Type 1 Polypurine Tract (PPT) Reduce the Rate of PPT Cleavage and Plus-Strand DNA Synthesis

机译：人类免疫缺陷病毒1型多嘌呤区（PPT）中的突变降低了PPT切割和正链DNA合成的速率

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摘要

Previously, we analyzed the effects of point mutations in the human immunodeficiency virus type 1 (HIV-1) polypurine tract (PPT) and found that some mutations affected both titer and cleavage specificity. We used HIV-1 vectors containing two PPTs and the D116N integrase active-site mutation in a cell-based assay to measure differences in the relative rates of PPT processing and utilization. The relative rates were measured by determining which of the two PPTs in the vector is used to synthesize viral DNA. The results indicate that mutations that have subtle effects on titer and cleavage specificity can have dramatic effects on rates of PPT generation and utilization.

机译：以前，我们分析了1型人类免疫缺陷病毒（HIV-1）多嘌呤道（PPT）中点突变的影响，发现某些突变会影响滴度和切割特异性。我们在基于细胞的试验中使用了含有两个PPT和D116N整合酶活性位点突变的HIV-1载体，以测量PPT加工和利用的相对速率的差异。通过确定载体中两种PPT中的哪一种用于合成病毒DNA来测量相对速率。结果表明，对滴度和切割特异性有微妙影响的突变可对PPT的产生和利用速率产生显着影响。

著录项

期刊名称 Journal of Virology
作者
M. J. McWilliams; J. G. Julias; S. H. Hughes;
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年(卷),期 2008(82),10
年度 2008
页码 5104–5108
总页数 5
原文格式 PDF
正文语种
中图分类人体病毒学（致病病毒）;病毒（滤过性病毒）;
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专利

1. In vitro analysis of the effects of mutations in the G-tract of the human immunodeficiency virus type 1 polypurine tract on RNase H cleavage specificity [J] . Jones FD, Hughes SH Virology . 2007,第2期

机译：人体免疫缺陷病毒1型多嘌呤道G道突变对RNase H切割特异性影响的体外分析
2. Using pyrrolo-deoxycytosine to probe RNA/DNA hybrids containing the human immunodeficiency virus type-1 3' polypurine tract [J] . Chandravanu Dash, Jason W. Rausch, Stuart F. J. Le Grice Nucleic Acids Research . 2004,第4期

机译：使用吡咯烷-脱氧胞嘧啶探查含有人免疫缺陷病毒1型3'多嘌呤束的RNA / DNA杂种
3. Using pyrrolo‐deoxycytosine to probe RNA/DNA hybrids containing the human immunodeficiency virus type‐1 3′ polypurine tract [J] . Chandravanu Dash, Jason W. Rausch, Stuart F. J. Le Grice Nucleic acids research . 2004,第4期

机译：使用吡咯菌-脱氧胞嘧啶探查含有人类免疫缺陷病毒1型3'多嘌呤束的RNA / DNA杂种
4. Prediction of human immunodeficiency virus type 1 drug resistance: Representation of target sequence mutational patterns via an n-grams approach [C] . Masso Majid 2012 IEEE International Conference on Bioinformatics and Biomedicine. . 2012

机译：预测人类1型免疫缺陷病毒耐药性：通过n-grams方法表示靶序列突变模式
5. Do nucleoside analog-resistance mutations increase the fidelity of human immunodeficiency virus type-1 (HIV-1) reverse transcriptase? [D] . Curr, Kenneth Andrew. 2003

机译：核苷类似物抗性突变是否增加了人类1型免疫缺陷病毒（HIV-1）逆转录酶的保真度？
6. Effect of Polypurine Tract (PPT) Mutations on Human Immunodeficiency Virus Type 1 Replication: a Virus with a Completely Randomized PPT Retains Low Infectivity [O] . Lesa R. Miles, Beth E. Agresta, Mahfuz B. Khan, 2005

机译：多嘌呤域（PPT）突变对人类免疫缺陷病毒1型复制的影响：具有完全随机PPT的病毒保持低感染性
7. Mutations in the Human Immunodeficiency Virus Type 1 Polypurine Tract (PPT) Reduce the Rate of PPT Cleavage and Plus-Strand DNA Synthesis▿ † [O] . McWilliams, M. J., Julias, J. G., Hughes, S. H. 2008

机译：人类免疫缺陷病毒1型多嘌呤区（PPT）中的突变降低了PPT切割的速率和正链DNA合成▿†
8. Mutations in the N-Terminal Region of Human Immunodeficiency Virus Type 1 MatrixProtein Block Intracellular Transport of the Gag Precursor [R] . Yuan, X., Yu, X., Lee, T. H., 1993

机译：人类免疫缺陷病毒1型基质蛋白的N-末端区突变阻断Gag前体的细胞内转运

Mutations in the Human Immunodeficiency Virus Type 1 Polypurine Tract (PPT) Reduce the Rate of PPT Cleavage and Plus-Strand DNA Synthesis

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