An optimal viral peptide recognized by CD8+ T cells binds very tightly to the restricting class I major histocompatibility complex protein on intact cells but not to the purified class I protein.

机译：CD8 + T细胞识别的最佳病毒肽与完整细胞上的限制性I类主要组织相容性复合蛋白紧密结合但不与纯化的I类蛋白紧密结合。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

CD8+ cytotoxic T lymphocytes recognize cell surface complexes formed by class I major histocompatibility complex (MHC-I) glycoproteins and antigenic peptides. We have identified a peptide nonamer (termed IV9) derived from the human immunodeficiency virus that is over a millionfold more active (at subpicomolar concentrations) than peptide analogues longer or shorter by one or two amino acid residues. Although IV9 does not detectably bind to isolated MHC-I molecules as measured by equilibrium dialysis, we quantitated its specific binding in unaltered form to MHC-I on intact cells. Less than 1% of cell surface MHC-I forms complexes with IV9, which suffices to trigger maximal cytotoxic T-lymphocyte activity. By contrast, a peptide dodecamer that includes the IV9 sequence and is active at micromolar concentrations does not bind to MHC-I on intact cells, raising the possibility that this longer peptide undergoes processing. Using stoichiometrically iodinated IV9 to obviate the ambiguities associated with trace labeling methods, we measured the dissociation kinetics of purified peptide/MHC-I complexes isolated by affinity chromatography and found these complexes to be exceedingly stable (t1/2 = 200-600 hr).

机译：CD8 +细胞毒性T淋巴细胞识别由I类主要组织相容性复合物（MHC-1）糖蛋白和抗原性肽形成的细胞表面复合物。我们已经鉴定出一种源自人免疫缺陷病毒的肽九聚体（称为IV9），其活性（在皮摩尔浓度下）比更长或更短一个或两个氨基酸残基的肽类似物高一百万倍。尽管通过平衡透析测量，IV9不能检测到与分离的MHC-1分子的结合，但我们以完整的形式定量了其与完整细胞上MHC-1的特异性结合。少于1％的细胞表面MHC-1与IV9形成复合物，足以触发最大的细胞毒性T淋巴细胞活性。相反，包括IV9序列并且在微摩尔浓度下有活性的十二肽肽不与完整细胞上的MHC-1结合，从而增加了这种较长肽进行加工的可能性。使用化学计量碘化的IV9消除了与痕量标记方法相关的歧义，我们测量了通过亲和色谱分离的纯化肽/ MHC-1复合物的解离动力学，发现这些复合物极其稳定（t1 / 2 = 200-600 hr）。

著录项

期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
T J Tsomides; B D Walker; H N Eisen;
展开▼
作者单位

展开▼
年(卷),期 1991(88),24
年度 1991
页码 11276–11280
总页数 5
原文格式 PDF
正文语种
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. CD40 Ligand-activated, antigen-specific B cells are comparable to mature dendritic cells in presenting protein antigens and major histocompatibility complex class I- and class II-binding peptides. [J] . Ahmadi T, Flies A, Efebera Y, Immunology: An Official Journal of the British Society for Immunology . 2008,第1期

机译：CD40配体激活的抗原特异性B细胞在呈递蛋白质抗原和主要的组织相容性复杂的I类和II类结合肽方面与成熟的树突状细胞相当。
2. Folding and assembly of major histocompatibility complex class I heterodimers in the endoplasmic reticulum of intact cells precedes the binding of peptide. [J] . J J Neefjes, G J H?mmerling, F Momburg The Journal of Experomental Medicine . 1993,第6期

机译：完整细胞内质网中主要组织相容性复杂的I类异源二聚体的折叠和组装先于肽的结合。
3. Regulatory CD4(+) T Cells Recognize Major Histocompatibility Complex Class II Molecule-Restricted Peptide Epitopes of Apolipoprotein B [J] . Kimura Takayuki, Kobiyama Kouji, Winkels Holger, Circulation: An Official Journal of the American Heart Association . 2018,第11期

机译：调节CD4（+）T细胞识别脂蛋白B的主要组织相容性复合体II类分子限制肽表位
4. Invariant chain regulates the major histocompatibility complex class II peptide repertoire of cell-based cancer vaccines. [C] . Olesya Y. Chornoguz, Lydia Grmai, Alexei Gapeev, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：不变链调节细胞基癌症疫苗的主要组织相容性复合体II类肽曲目。
5. Dynamics of peptides bound to class I major histocompatibility complex proteins: Implications for T-cell receptor recognition in the immune response [D] . Insaidoo, Francis Kobina 2010

机译：与I类主要组织相容性复杂蛋白结合的肽的动力学：免疫应答中T细胞受体识别的意义
6. CD40 Ligand-activated antigen-specific B cells are comparable to mature dendritic cells in presenting protein antigens and major histocompatibility complex class I- and class II-binding peptides [O] . Tahamtan Ahmadi, Amanda Flies, Yvonne Efebera, 2008

机译：CD40配体激活的抗原特异性B细胞在呈递蛋白质抗原和主要的组织相容性复杂的I类和II类结合肽方面可与成熟的树突状细胞媲美
7. An optimal viral peptide recognized by CD8+ T cells binds very tightly to the restricting class I major histocompatibility complex protein on intact cells but not to the purified class I protein. [O] . Tsomides, T J, Walker, B D, Eisen, H N 1991

机译：CD8 + T细胞识别的最佳病毒肽与完整细胞上的限制性I类主要组织相容性复合蛋白紧密结合，但不与纯化的I类蛋白紧密结合。

An optimal viral peptide recognized by CD8+ T cells binds very tightly to the restricting class I major histocompatibility complex protein on intact cells but not to the purified class I protein.

摘要

著录项

相似文献

相关主题

期刊订阅