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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunit.
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Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunit.

机译：通过结合亚基的定点诱变改变了维毒素从Gal Gal 1-4Gal到GalNAc beta 1-3Gal alpha 1-4Gal的碳水化合物结合特异性反之亦然。

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摘要

Verotoxin 1 (VT-1) and Shiga-like toxin II (SLT-II) bind to the glycosphingolipid (GSL), globotriaosylceramide (Gb3), whereas pig edema disease toxin (VTE) binds to globotetraosylceramide (Gb4) and to a lesser degree Gb3. Amino acids important in the GSL binding specificity of VT-1 and VTE have been identified by site-directed mutagenesis. One mutation, Asp-18----Asn, in VT-1 resulted in binding to Gb4 in addition to Gb3 in a manner similar to VTE. Several mutations in VTE resulted in the complete loss of GSL binding; however, one mutation resulted in a change in the GSL binding specificity of the VTE B subunit. The double mutation Gln-64----Glu and Lys-66----Gln (designated GT3) caused a selective loss of Gb4 binding, effectively changing the binding phenotype from VTE to VT-1. Both wild-type VTE and GT3 were purified to homogeneity and binding kinetics in vitro were determined with purified GSLs from human kidney. The cell cytotoxicity spectrum of the mutant toxin was also found to be altered in comparison with VTE. These changes were consistent with the GSL content of the target cells.
机译：Verotoxin 1（VT-1）和Shiga-like毒素II（SLT-II）与糖鞘脂（GSL），globotriaosylceramide（Gb3）结合，而猪水肿病毒素（VTE）与globotetraosylceramide（Gb4）结合并且结合程度较小Gb3。通过定点诱变已经鉴定出对VT-1和VTE的GSL结合特异性重要的氨基酸。 VT-1中的一个突变Asp-18-Asn导致以与VTE相似的方式与Gb4以及Gb3结合。 VTE中的几个突变导致GSL结合的完全丧失。然而，一个突变导致VTE B亚基的GSL结合特异性改变。双重突变Gln-64 ---- Glu和Lys-66 ---- Gln（指定为GT3）导致Gb4结合的选择性丧失，有效地将结合表型从VTE改变为VT-1。将野生型VTE和GT3均纯化至均一，并使用来自人肾脏的纯化GSL确定体外结合动力学。与VTE相比，突变毒素的细胞毒性谱也被发现改变。这些变化与靶细胞的GSL含量一致。

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