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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Direct vibrational structure of protein metal-binding sites from near-infrared Yb3+ vibronic side band spectroscopy.
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Direct vibrational structure of protein metal-binding sites from near-infrared Yb3+ vibronic side band spectroscopy.

机译：蛋白质金属结合位点的直接振动结构来自近红外Yb3 +振动电子边带光谱法。

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摘要

Near-infrared Yb3+ vibronic side band (VSB) spectroscopy is used to obtain structural information of metal binding sites in metalloproteins. This technique provides a selective "IR-like" vibrational spectrum of those ligands chelated to the Yb3+ ion. VSB spectra of various model complexes of Yb3+ representing different ligand types were studied to provide references for the VSB spectra of Yb(3+)-reconstituted metalloproteins. Ca2+ in the calcium-binding protein parvalbumin and Fe3+ in the iron-transporting protein transferrin were replaced with Yb3+. The fluorescence of Yb3+ reconstituted into these two proteins exhibits weak VSBs whose energy shifts, with respect to the main 2F5/2-->2F7/2 Yb3+ electronic transition, represent the vibrational frequencies of the Yb3+ ligands. The chemical nature of the ligands of the Yb3+ in these proteins, as deduced by the observed VSB frequencies, is entirely in agreement with their known crystal structures. For transferrin, replacement of the 12CO3(2-) metal counterion with 13CO3(2-) yielded the expected isotopic shift for the VSBs corresponding to the carbonate vibrational modes. This technique demonstrates enormous potential in elucidating the localized structure of metal binding sites in proteins.
机译：近红外Yb3 +振动电子边带（VSB）光谱用于获得金属蛋白中金属结合位点的结构信息。该技术提供了与Yb3 +离子螯合的那些配体的选择性“类IR”振动光谱。研究了代表不同配体类型的Yb3 +的各种模型复合物的VSB光谱，为Yb（3+）重构的金属蛋白的VSB光谱提供参考。钙结合蛋白小白蛋白中的Ca2 +和铁转运蛋白转铁蛋白中的Fe3 +被Yb3 +取代。重构为这两种蛋白质的Yb3 +的荧光表现出弱的VSB，其相对于主要2F5 / 2-> 2F7 / 2 Yb3 +电子跃迁的能量转移代表Yb3 +配体的振动频率。由观察到的VSB频率推论，这些蛋白质中Yb3 +配体的化学性质与它们的已知晶体结构完全一致。对于运铁蛋白，用13CO3（2-）取代12CO3（2-）金属抗衡离子可产生对应于碳酸盐振动模式的VSB的预期同位素位移。该技术在阐明蛋白质中金属结合位点的局部结构方面显示出巨大潜力。

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