首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Phytochelatin production by marine phytoplankton at low free metal ion concentrations: laboratory studies and field data from Massachusetts Bay.
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Phytochelatin production by marine phytoplankton at low free metal ion concentrations: laboratory studies and field data from Massachusetts Bay.

机译:低游离金属离子浓度下海洋浮游植物产生的植物螯合素:来自马萨诸塞湾的实验室研究和田间数据。

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摘要

Phytochelatins are small metal-binding polypeptides synthesized by algae in response to high metal concentrations. Using a very sensitive HPLC method, we have quantified phytochelatins from phytoplankton in laboratory cultures at environmentally relevant metal concentrations and in marine field samples. Intracellular concentrations of phytochelatin, in the diatom Thalassiosira weissflogii, exhibit a distinct dose-response relation with free Cd2+ concentration in the medium--not with total Cd(2+)--and are detectable even when the free Cd2+ concentration is less than 1 pM. In Massachusetts Bay, phytochelatin levels (normalized to chlorophyll a) in the particulate fraction are similar to those measured in laboratory cultures exposed to picomolar free Cd2+ concentrations and exhibit a decreasing seaward trend. Incubations of natural samples with added Cd2+ confirmed the induction of the peptides by this metal. Ambient phytochelatin concentrations thus appear to provide a measure of the metal stress resulting from the complex mixture of trace metals and chelators in natural waters.
机译:植物螯合素是藻类响应高金属浓度而合成的小金属结合多肽。使用非常灵敏的HPLC方法,我们在实验室培养物中以环境相关的金属浓度和海洋样本中的浮游植物中的植物螯合素进行了定量。硅藻Thalassiosira weissflogii中植物螯合素的细胞内浓度与培养基中的游离Cd2 +浓度表现出明显的剂量反应关系-而与总Cd(2+)无关-即使游离Cd2 +浓度小于1时也可以检测到下午。在马萨诸塞湾,颗粒级分中植物螯合素水平(标准化为叶绿素a)与暴露于皮摩尔游离Cd2 +浓度的实验室培养物中测得的相似,并显示出下降趋势。添加了Cd2 +的天然样品的孵育证实了这种金属对肽的诱导。因此,植物螯合素的环境浓度似乎可以衡量由天然水中微量金属和螯合剂的复杂混合物引起的金属胁迫。

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