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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Redox regulation of surface protein thiols: Identification of integrin α-4 as a molecular target by using redox proteomics
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Redox regulation of surface protein thiols: Identification of integrin α-4 as a molecular target by using redox proteomics

机译：表面蛋白硫醇的氧化还原调节：通过使用氧化还原蛋白质组学鉴定整联蛋白α-4作为分子靶标

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摘要

Thiols affect a variety of cell functions, an effect known as redox regulation. We show here that treatment (1–2 h) of cells with 0.1–5 mM N-acetyl-l-cysteine (NAC) increases surface protein thiol expression in human peripheral blood mononuclear cells. This effect is not associated with changes in cellular glutathione (GSH) and is also observed with a non-GSH precursor thiol N-acetyl-d-cysteine or with GSH itself, which is not cell-permeable, suggesting a direct reducing action. NAC did not augment protein SH in the cytosol, indicating that they are already maximally reduced under normal, nonstressed, conditions. By using labeling with a non permeable, biotinylated SH reagent followed by two-dimensional gel electrophoresis and analysis by MS, we identified some of the proteins associated with the membrane that are reduced by NAC. These proteins include the following: integrin α-4, myosin heavy chain (nonmuscle type A), myosin light-chain alkali (nonmuscle isoform), and β-actin. NAC pretreatment augmented integrin α-4-dependent fibronectin adhesion and aggregation of Jurkat cells without changing its expression by fluorescence-activated cell sorter, suggesting that reduction of surface disulfides can affect proteins function. We postulate that some of the activities of NAC or other thiol antioxidants may not only be due to free radical scavenging or increase of intracellular GSH and subsequent effects on transcription factors, but could modify the redox state of functional membrane proteins with exofacial SH critical for their activity.
机译：硫醇影响多种细胞功能，这种作用称为氧化还原调节。我们在这里表明，用0.1–5 mM N-乙酰基-1-半胱氨酸（NAC）处理细胞（1-2小时）会增加人外周血单核细胞中表面蛋白硫醇的表达。该作用与细胞谷胱甘肽（GSH）的变化无关，并且在非GSH前体硫醇N-乙酰基-d-半胱氨酸或与GSH本身（细胞不可渗透）中也观察到，表明存在直接还原作用。 NAC不会增加胞质溶胶中的蛋白质SH，表明它们在正常，无压力的条件下已被最大程度地还原。通过使用不可渗透的生物素化SH试剂进行标记，然后进行二维凝胶电泳和MS分析，我们鉴定了与膜相关的一些蛋白质，这些蛋白质被NAC还原。这些蛋白质包括：整联蛋白α-4，肌球蛋白重链（非肌肉A型），肌球蛋白轻链碱（非肌肉同种型）和β-肌动蛋白。 NAC预处理可增强Jurkat细胞的整合素α-4依赖性纤连蛋白粘附和聚集，而无需通过荧光激活细胞分选仪改变其表达，这表明表面二硫化物的减少可影响蛋白质功能。我们推测，NAC或其他巯基抗氧化剂的某些活性可能不仅是由于自由基清除或细胞内GSH的增加以及对转录因子的后续影响，而且可能会改变功能性膜蛋白的氧化还原状态，而这些蛋白对它们的面颊SH至关重要活动。

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