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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >From the Cover: Borrelia burgdorferi σ54 is required for mammalian infection and vector transmission but not for tick colonization
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From the Cover: Borrelia burgdorferi σ54 is required for mammalian infection and vector transmission but not for tick colonization

机译：从封面开始：哺乳动物感染和媒介传播需要伯氏疏螺旋体σ54但壁虱定殖则不需要

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摘要

Previous studies have shown that a σ54–σS cascade regulates the expression of a few key lipoproteins in Borrelia burgdorferi, the agent of Lyme disease. Here, we demonstrate that these sigma factors, both together and independently, regulate a much more extensive number of genes and cellular processes. Microarray analyses of σ54 and σS mutant strains identified 305 genes regulated by σ54 and 145 regulated by σS, whereas the σ54–σS regulatory cascade appears to control 48 genes in B. burgdorferi. In silico analyses revealed that nearly 80% of genes with altered expression in the σ54 mutant were linked to potential σ54-dependent promoters. Many σ54-regulated genes are expressed in vivo, and through genetic complementation of the mutant, we demonstrated that σ54 was required by B. burgdorferi to infect mammals. Surprisingly, σ54 mutants were able to infect Ixodes scapularis ticks and be maintained for at least 24 wk after infection, suggesting the σ54–σS regulatory network was not involved in long-term survival in ticks. However, σ54 mutants did not enter the salivary glands during tick feeding, indicating that σ54-regulated genes were involved in the transmission process.
机译：先前的研究表明，σ 54 –σ S 级联调节莱姆病病原体伯氏疏螺旋体中一些关键脂蛋白的表达。在这里，我们证明了这些sigma因子共同且独立地调节着数量更多的基因和细胞过程。对σ 54 和σ S 突变菌株的微阵列分析鉴定了305个受σ 54 调控的基因和145个受σ S ，而σ 54 –σ S 调控级联似乎控制了B. burgdorferi中的48个基因。在计算机分析中发现，在σ 54 突变体中表达改变的基因中有近80％与潜在的σ 54 依赖性启动子相关。体内表达了许多受σ 54 调控的基因，通过突变体的遗传互补，我们证明了伯氏疏螺旋体需要σ 54 感染哺乳动物。令人惊讶的是，σ 54 突变体能够感染肩小I，并在感染后至少维持了24 wk，这表明σ 54 –σ S 监管网络没有参与survival的长期生存。然而，tick饲养期间σ 54 突变体并未进入唾液腺，这表明σ 54 调控的基因参与了传播过程。

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