• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Genomic imprinting of XX spermatogonia and XX oocytes recovered from XX↔XY chimeric testes
【2h】

Genomic imprinting of XX spermatogonia and XX oocytes recovered from XX↔XY chimeric testes

机译：从XX↔XY嵌合睾丸中回收的XX精原细胞和XX卵母细胞的基因组印迹

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We produced XX↔XY chimeras by using embryos whose X chromosomes were tagged with EGFP (X*), making the fluorescent green female (XX*) germ cells easily distinguishable from their nonfluorescent male (XY) counterparts. Taking advantage of tagging with EGFP, the XX* “prospermatogonia” were isolated from the testes, and the status of their genomic imprinting was examined. It was shown that these XX cells underwent a paternal imprinting, despite their chromosomal constitution. As previously indicated in sex-reversal XXsxr testes, we also found a few green XX* germ cells developed as “eggs” within the seminiferous tubules of XX*↔XY chimeric testes. These cells were indistinguishable from XX* prospermatogonia at birth but resumed oogenesis in a testicular environment. The biological nature of the “testicular eggs” was examined by recovering the eggs from chimeric testes. The testicular eggs not only formed an egg-specific structure, the zona pellucida, but also were able to fuse with sperm. The collected testicular eggs were indicated to undergo maternal imprinting, despite the testicular environment. The genomic imprinting did not always follow the environmental conditions of where the germ cells resided; rather, it was defined by the sex that was chosen by the germ cells at early embryonic stage.
机译：我们通过使用X染色体标记有EGFP（X *）的胚胎生产XX↔XY嵌合体，从而使荧光绿色雌性（XX *）生殖细胞易于与非荧光雄性（XY）对应物区分开。利用EGFP标记技术，从睾丸中分离出XX *“上生菌”，并检查了其基因组印迹的状态。结果表明，尽管它们具有染色体组成，但这些XX细胞仍具有父系印记。正如先前在性逆转XXsxr睾丸中指出的那样，我们还在XX *↔XY嵌合睾丸的生精小管中发现了一些绿色的XX *绿色生殖细胞，形成了“卵”。这些细胞在出生时与XX *前列腺素原没有区别，但在睾丸环境中恢复了卵子形成。通过从嵌合睾丸中回收卵来检查“睾丸卵”的生物学特性。睾丸卵不仅形成卵特异性的结构，透明带，而且还能够与精子融合。尽管有睾丸环境，但收集到的睾丸卵仍显示有母体印记。基因组印记并不总是遵循生殖细胞所处的环境条件。相反，它是由胚胎早期生殖细胞选择的性别定义的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号