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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Physical limits to spatial resolution of optical recording: Clarifying the spatial structure of cortical hypercolumns
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Physical limits to spatial resolution of optical recording: Clarifying the spatial structure of cortical hypercolumns

机译：光学记录的空间分辨率的物理限制：阐明皮质超柱的空间结构

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摘要

Neurons in macaque primary visual cortex are spatially arranged by their global topographic position and in at least three overlapping local modular systems: ocular dominance columns, orientation pinwheels, and cytochrome oxidase (CO) blobs. Individual neurons in the blobs are not tuned to orientation, and populations of neurons in the pinwheel center regions show weak orientation tuning, suggesting a close relation between pinwheel centers and CO blobs. However, this hypothesis has been challenged by a series of optical recording experiments. In this report, we show that the statistical error associated with photon scatter and absorption in brain tissue combined with the blurring introduced by the optics of the imaging system has typically been in the range of 250 μm. These physical limitations cause a systematic error in the location of pinwheel centers because of the vectorial nature of these patterns, such that the apparent location of a pinwheel center measured by optical recording is never (on average) in the correct in vivo location. The systematic positional offset is ≈116 μm, which is large enough to account for the claimed misalignment of CO blobs and pinwheel centers. Thus, optical recording, as it has been used to date, has insufficient spatial resolution to accurately locate pinwheel centers. The earlier hypothesis that CO blobs and pinwheel centers are coterminous remains the only hypothesis currently supported by reliable observation.
机译：猕猴初级视觉皮层中的神经元在空间上按其整体地形位置排列，并在至少三个重叠的局部模块化系统中排列：眼优势柱，定向风车和细胞色素氧化酶（CO）斑点。斑点中的单个神经元未调整到方向，而风车中心区域中的神经元种群显示弱的方向调整，表明风车中心与CO斑点之间的紧密关系。但是，这一假设受到了一系列光学记录实验的挑战。在此报告中，我们显示与脑组织中的光子散射和吸收以及成像系统的光学器件引入的模糊相结合的统计误差通常在250μm范围内。由于这些模式的矢量性质，这些物理限制导致风车中心位置的系统误差，从而使得通过光学记录测量的风车中心的外观位置永远不会（平均）位于正确的体内位置。系统的位置偏移约为116微米，足以解决所声称的CO斑点和风车中心未对准的问题。因此，迄今为止所使用的光学记录的空间分辨率不足以精确地定位风车中心。 CO斑点和风车中心是连续的较早的假设仍然是目前唯一得到可靠观察支持的假设。

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