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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Bacteriorhodopsin (bR) as an electronic conduction medium: Current transport through bR-containing monolayers
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Bacteriorhodopsin (bR) as an electronic conduction medium: Current transport through bR-containing monolayers

机译：细菌视紫红质（bR）作为电子传导介质：通过含bR单层的电流传输

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摘要

Studying electron transport (ET) through proteins is hampered by achieving reproducible experimental configurations, particularly electronic contacts to the proteins. The transmembrane protein bacteriorhodopsin (bR), a natural light-activated proton pump in purple membranes of Halobacterium salinarum, is well studied for biomolecular electronics because of its sturdiness over a wide range of conditions. To date, related studies of dry bR systems focused on photovoltage generation and photoconduction with multilayers, rather than on the ET ability of bR, which is understandable because ET across 5-nm-thick, apparently insulating membranes is not obvious. Here we show that electronic current passes through bR-containing artificial lipid bilayers in solid “electrode–bilayer–electrode” structures and that the current through the protein is more than four orders of magnitude higher than would be estimated for direct tunneling through 5-nm, water-free peptides. We find that ET occurs only if retinal or a close analogue is present in the protein. As long as the retinal can isomerize after light absorption, there is a photo-ET effect. The contribution of light-driven proton pumping to the steady-state photocurrents is negligible. Possible implications in view of the suggested early evolutionary origin of halobacteria are noted.
机译：通过实现可重现的实验配置，尤其是与蛋白质的电子接触，阻碍了研究通过蛋白质的电子传输（ET）。跨膜蛋白细菌视紫红质（bR）是盐沼盐杆菌紫色膜中的一种天然光活化质子泵，由于其在各种条件下的坚固性，因此对生物分子电子学进行了充分的研究。迄今为止，干式bR系统的相关研究集中于多层的光电压产生和光电导，而不是bR的ET能力，这是可以理解的，因为横跨5纳米厚，明显绝缘膜的ET并不明显。在这里，我们表明，电流通过固体“电极-双层-电极”结构中含bR的人工脂质双层，并且通过蛋白质的电流比通过5 nm直接隧穿的估计电流高四个数量级。 ，无水肽。我们发现仅当蛋白质中存在视网膜或紧密类似物时才发生ET。只要视网膜在光吸收后可以异构化，就存在光ET效应。光驱动质子泵对稳态光电流的贡献可忽略不计。考虑到建议的卤代细菌的早期进化起源，可能的含义。

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