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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >From the Cover: Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination
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From the Cover: Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination

机译：从封面开始：基于水稻的粘膜疫苗是冷链和无针疫苗的全球战略

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Capable of inducing antigen-specific immune responses in both systemic and mucosal compartments without the use of syringe and needle, mucosal vaccination is considered ideal for the global control of infectious diseases. In this study, we developed a rice-based oral vaccine expressing cholera toxin B subunit (CTB) under the control of the endosperm-specific expression promoter 2.3-kb glutelin GluB-1 with codon usage optimization for expression in rice seed. An average of 30 μg of CTB per seed was stored in the protein bodies, which are storage organelles in rice. When mucosally fed, rice seeds expressing CTB were taken up by the M cells covering the Peyer's patches and induced CTB-specific serum IgG and mucosal IgA antibodies with neutralizing activity. When expressed in rice, CTB was protected from pepsin digestion in vitro. Rice-expressed CTB also remained stable and thus maintained immunogenicity at room temperature for >1.5 years, meaning that antigen-specific mucosal immune responses were induced at much lower doses than were necessary with purified recombinant CTB. Because they require neither refrigeration (cold-chain management) nor a needle, these rice-based mucosal vaccines offer a highly practical and cost-effective strategy for orally vaccinating large populations against mucosal infections, including those that may result from an act of bioterrorism.
机译：能够在不使用注射器和针头的情况下在全身和粘膜区室中诱导抗原特异性免疫应答，粘膜疫苗被认为是全球控制传染病的理想选择。在这项研究中，我们开发了一种基于水稻的口服疫苗，可在胚乳特异性表达启动子2.3-kb谷蛋白GluB-1的控制下表达霍乱毒素B亚基（CTB），并在水稻种子中进行密码子使用优化。每个种子中平均有30μg的CTB存储在蛋白质体内，这些蛋白质是水稻中的存储细胞器。粘膜喂养时，表达CTB的水稻种子被覆盖Peyer斑块的M细胞吸收，并诱导CTB特异性血清IgG和具有中和活性的粘膜IgA抗体。当在水稻中表达时，CTB在体外不受胃蛋白酶消化。水稻表达的CTB也保持稳定，因此在室温下保持免疫原性> 1.5年，这意味着与纯化的重组CTB相比，以更低的剂量诱导了抗原特异性粘膜免疫反应。由于这些基于水稻的粘膜疫苗既不需要冷藏（冷链管理）也不需要注射针，因此提供了一种非常实用且具有成本效益的策略，可以对大量人群进行口服疫苗接种以预防粘膜感染，包括那些可能由生物恐怖行为引起的感染。

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