Transcription factor TFIIF is not required for initiation by RNA polymerase II but it is essential to stabilize transcription factor TFIIB in early elongation complexes

机译：RNA聚合酶II不需要转录因子TFIIF来启动但是在早期延伸复合物中稳定转录因子TFIIB至关重要

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Transcription factors TFIIB and TFIIF are both required for RNA polymerase II preinitiation complex (PIC) assembly, but their roles at and downstream of initiation are not clear. We now show that TFIIF phosphorylated by casein kinase 2 remains competent to support PIC assembly but is not stably retained in the PIC. PICs completely lacking TFIIF are not defective in initiation or subsequent promoter clearance, demonstrating that TFIIF is not required for initiation or clearance. Lack of TFIIF in the PIC reduces transcription levels at some promoters, coincident with reduced retention of TFIIB. TFIIB is normally associated with the early elongation complex and is only destabilized at +12 to +13. However, if TFIIF is not retained in the PIC, TFIIB can be lost immediately after initiation. TFIIF therefore has an important role in stabilizing TFIIB within the PIC and after transcription initiates.

机译：转录因子TFIIB和TFIIF都是RNA聚合酶II预起始复合物（PIC）组装所必需的，但它们在起始和下游的作用尚不清楚。我们现在显示酪蛋白激酶2磷酸化的TFIIF仍然能够支持PIC组装，但不能稳定地保留在PIC中。完全缺乏TFIIF的PIC在启动或启动子清除后没有缺陷，表明启动或清除不需要TFIIF。 PIC中缺乏TFIIF会降低某些启动子的转录水平，同时降低TFIIB的保留。 TFIIB通常与早期伸长复合物有关，仅在+12至+13时不稳定。但是，如果TFIIF没有保留在PIC中，则TFIIB可能会在启动后立即丢失。因此，TFIIF在稳定PIC中以及转录开始后的TFIIB中起着重要作用。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
Pavel Čabart; Andrea Újvári; Mahadeb Pal; Donal S. Luse;
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作者单位

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年(卷),期 2011(108),38
年度 2011
页码 15786-15791
总页数 6
原文格式 PDF
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关键词
general transcription factors transcript initiation;

机译：一般转录因子;转录起始;

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专利

1. The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation [J] . Khoo Seok-Kooi, Wu Chih-Chien, Lin Yu-Chun, Nucleic Acids Research . 2018,第3期

机译：RNA聚合酶III的TFIIE相关的RPC82亚基与TFIIB相关转录因子BRF1和用于转录起始的聚合酶裂隙相互作用
2. The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation [J] . Seok-Kooi Khoo, Chih-Chien Wu, Hung-Ta Chen, Nucleic acids research . 2017,第3期

机译：RNA聚合酶III的TFIIE相关的Rpc82亚基与TFIIB相关的转录因子Brf1相互作用，聚合酶裂开
3. Dissection of transcription factor TFIIF functional domains required for initiation and elongation. [J] . Tan S, Conaway RC, Conaway JW Proceedings of the National Academy of Sciences of the United States of America . 1995,第13期

机译：解剖起始和延伸所需的转录因子TFIIF功能域。
4. Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB [C] . Finn Werner, Simone Wiesler, Sven Nottebaum Biochemical Society Symposium . 2006

机译：通过基础转录因子TFB / TFIIB调节RNA聚合酶核心功能
5. Regulation of transcription elongation via interactions of RNA polymerase with sequence elements and accessory factors [D] . Pearson, Erika L. 2008

机译：通过RNA聚合酶与序列元素和附带因子相互作用的转录伸长
6. The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation [O] . Seok-Kooi Khoo, Chih-Chien Wu, Yu-Chun Lin, 2018

机译：RNA聚合酶III的与TFIIE相关的Rpc82亚基与TFIIB相关的转录因子Brf1相互作用并且聚合酶裂开
7. Transcription factor TFIIF is not required for initiation by RNA polymerase II, but it is essential to stabilize transcription factor TFIIB in early elongation complexes [O] . Čabart, Pavel, Újvári, Andrea, Pal, Mahadeb, 2011

机译：RNA聚合酶II不需要转录因子TFIIF，但是在早期延伸复合物中稳定转录因子TFIIB是必不可少的

Transcription factor TFIIF is not required for initiation by RNA polymerase II but it is essential to stabilize transcription factor TFIIB in early elongation complexes

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