PNAS Plus: Heterotrimeric G protein β1γ2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane

机译：PNAS Plus：异源三聚体G蛋白β1γ2亚基在模型膜上与G蛋白偶联受体激酶2（GRK2）形成复合物后会改变方向

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摘要

Few experimental techniques can assess the orientation of peripheral membrane proteins in their native environment. Sum Frequency Generation (SFG) vibrational spectroscopy was applied to study the formation of the complex between G protein-coupled receptor (GPCR) kinase 2 (GRK2) and heterotrimeric G protein β1γ2 subunits (Gβγ) at a lipid bilayer, without any exogenous labels. The most likely membrane orientation of the GRK2-Gβγ complex differs from that predicted from the known protein crystal structure, and positions the predicted receptor docking site of GRK2 such that it would more optimally interact with GPCRs. Gβγ also appears to change its orientation after binding to GRK2. The developed methodology is widely applicable for the study of other membrane proteins in situ.

机译：很少有实验技术可以评估其天然环境中外周膜蛋白的方向。应用总频率生成（SFG）振动光谱法研究了在脂质双层中G蛋白偶联受体（GPCR）激酶2（GRK2）和异三聚G蛋白β1γ2亚基（Gβγ）之间没有任何外源标记的复合物的形成。 GRK2-Gβγ复合物最可能的膜取向与已知蛋白质晶体结构所预测的不同，并且将所预测的GRK2受体对接位点定位，从而使其与GPCR相互作用更佳。 Gβγ与GRK2结合后也似乎改变了其方向。所开发的方法可广泛用于其他原位膜蛋白的研究。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
Andrew P. Boughton; Pei Yang; Valerie M. Tesmer; Bei Ding; John J. G. Tesmer; Zhan Chen;
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年(卷),期 2011(108),37
年度 2011
页码 E667-E673
总页数 7
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1. Heterotrimeric G protein β1γ2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane [J] . Andrew P. Boughton, Pei Yang, Valerie M. Tesmer, Proceedings of the National Academy of Sciences of the United States of America . 2011,第37期

机译：异三聚体G蛋白β1γ2亚基在模型膜上与G蛋白偶联受体激酶2（GRK2）形成复合物后改变方向
2. Effect of Lipid Composition on the Membrane Orientation of the G Protein-Coupled Receptor Kinase 2-G beta(1)gamma(2) Complex [J] . Yang Pei, Homan Kristoff T., Li Yaoxin, Biochemistry . 2016,第20期

机译：脂质成分对G蛋白偶联受体激酶2-G beta（1）gamma（2）复合物的膜取向的影响。
3. G protein-coupled receptor kinase 2 (GRK2) is a Rho-activated scaffold protein for the ERK MAP kinase cascade [J] . Robinson J.D., Pitcher J.A. Cellular Signalling . 2013,第12期

机译：G蛋白偶联受体激酶2（GRK2）是ERK MAP激酶级联反应的Rho激活支架蛋白
4. G PROTEIN-COUPLED RECEPTOR KINASE TYPE 4 (GRK4) GENE VARIANTS AND RESPONSE TO ANGIOTENSIN II TYPE 1 RECEPTOR BLOCKER THERAPY [C] . Hironobu Sanada, Junichi Yatabe, Midori Sasaki Yatabe, 9th International Symposium on Salt(第九届世界盐业大会）论文集 . 2009

机译：G蛋白偶联的4型受体激酶（GRK4）基因变异和对血管紧张素II 1型受体阻滞剂的反应
5. G Protein-Coupled Receptor Kinase 2 (GRK2) and Toll-like Receptors as Regulators of Central Sensitization in Fibromyalgia and Chronic Temporomandibular Disorders [D] . Angkanawaraphan, Lalilta. 2018

机译：G蛋白偶联受体激酶2（GRK2）和Toll样受体作为纤维肌痛和慢性颞下颌关节疾病中枢敏化的调节剂。
6. Effect of Lipid Composition on Membrane Orientation of the G protein-coupled Receptor Kinase 2-Gβ1γ2 Complex [O] . Pei Yang, Kristoff T. Homan, Yaoxin Li, -1

机译：脂质成分对G蛋白偶联受体激酶2-Gβ1γ2复合物膜取向的影响
7. Heterotrimeric G protein β1γ2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane [O] . Boughton, Andrew P., Yang, Pei, Tesmer, Valerie M., 2011

机译：异三聚体G蛋白β1γ2亚基在模型膜上与G蛋白偶联受体激酶2（GRK2）形成复合物后改变方向

PNAS Plus: Heterotrimeric G protein β1γ2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane

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