• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Measuring collective transport by defined numbers of processive and nonprocessive kinesin motors
【2h】

Measuring collective transport by defined numbers of processive and nonprocessive kinesin motors

机译：通过定义数量的渐进式和非渐进式驱动电机来测量集体运输

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Intracellular transport is thought to be achieved by teams of motor proteins bound to a cargo. However, the coordination within a team remains poorly understood as a result of the experimental difficulty in controlling the number and composition of motors. Here, we developed an experimental system that links together defined numbers of motors with defined spacing on a DNA scaffold. By using this system, we linked multiple molecules of two different types of kinesin motors, processive kinesin-1 or nonprocessive Ncd (kinesin-14), in vitro. Both types of kinesins markedly increased their processivities with motor number. Remarkably, despite the poor processivity of individual Ncd motors, the coupling of two Ncd motors enables processive movement for more than 1 μm along microtubules (MTs). This improvement was further enhanced with decreasing spacing between motors. Force measurements revealed that the force generated by groups of Ncd is additive when two to four Ncd motors work together, which is much larger than that generated by single motors. By contrast, the force of multiple kinesin-1s depends only weakly on motor number. Numerical simulations and single-molecule unbinding measurements suggest that this additive nature of the force exerted by Ncd relies on fast MT binding kinetics and the large drag force of individual Ncd motors. These features would enable small groups of Ncd motors to crosslink MTs while rapidly modulating their force by forming clusters. Thus, our experimental system may provide a platform to study the collective behavior of motor proteins from the bottom up.
机译：细胞内运输被认为是通过与货物结合的运动蛋白团队来实现的。但是，由于在控制电动机的数量和组成方面存在实验上的困难，因此团队内部的协调性仍然知之甚少。在这里，我们开发了一个实验系统，该系统将DNA支架上定义数量的电动机和定义的间距链接在一起。通过使用此系统，我们在体外连接了两种不同类型的驱动蛋白驱动器（进行性驱动蛋白1或非进行性Ncd（驱动蛋白14））的多个分子。两种类型的驱动蛋白均显着增加其运动能力与运动次数。值得注意的是，尽管单个Ncd电机的加工性能很差，但两个Ncd电机的耦合使沿微管（MTs）的加工运动超过1μm。随着电机间距的减小，这种改进进一步增强。力的测量显示，当两到四个Ncd电动机一起工作时，由Ncd组产生的力是相加的，比单个电动机产生的力大得多。相比之下，多种驱动蛋白1s的作用力仅微弱地取决于运动数。数值模拟和单分子解离测量表明，Ncd施加的力的这种累加性质依赖于快速MT结合动力学和单个Ncd马达的大阻力。这些功能将使Ncd电机小组能够在通过形成簇快速调节其力的同时使MT交联。因此，我们的实验系统可以提供一个平台，从下至上研究运动蛋白的集体行为。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号